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Catalog Number Size Price
LS-C313406-10 10 µg $318 
LS-C313406-100 100 µg (0.5 mg/ml) $470 
IL18 Antibody - Western blot analysis of IL18 using anti-IL18 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat spleen tissue lysates. Lane 4: rat liver tissue lysates. Lane 5: rat testis tissue lysates. Lane 6: rat RH35 lysates. Lane 7: rat PC-12 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL18 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL18 at approximately 22KD. The expected band size for IL18 is at 22KD.
IL18 Antibody - WB of IL18 antibody. All lanes: Anti-IL18 at 0.5ug/ml. Lane 1: Rat Spleen Tissue Lysate at 40ug. Lane 2: Rat Thymus Tissue Lysate at 40ug. Lane 3: PC12 Whole Cell Lysate at 40ug. Predicted bind size: 22KD. Observed bind size: 22KD.
IL18 Antibody - Western blot analysis of IL18 using anti-IL18 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat spleen tissue lysates. Lane 4: rat liver tissue lysates. Lane 5: rat testis tissue lysates. Lane 6: rat RH35 lysates. Lane 7: rat PC-12 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL18 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL18 at approximately 22KD. The expected band size for IL18 is at 22KD.
IL18 Antibody - WB of IL18 antibody. All lanes: Anti-IL18 at 0.5ug/ml. Lane 1: Rat Spleen Tissue Lysate at 40ug. Lane 2: Rat Thymus Tissue Lysate at 40ug. Lane 3: PC12 Whole Cell Lysate at 40ug. Predicted bind size: 22KD. Observed bind size: 22KD.
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Polyclonal Rabbit anti‑Rat IL18 Antibody (aa37‑194, WB) LS‑C313406

Polyclonal Rabbit anti‑Rat IL18 Antibody (aa37‑194, WB) LS‑C313406

Note: This antibody replaces LS-C388669
Antibody:
IL18 Rabbit anti-Rat Polyclonal (aa37-194) Antibody
Application:
WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313406-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IL18 Rabbit anti-Rat Polyclonal (aa37-194) Antibody
Application:
WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified

Specifications

Description
IL18 antibody LS-C313406 is an unconjugated rabbit polyclonal antibody to rat IL18 (aa37-194). Validated for ELISA and WB.
Target
Rat IL18
Synonyms
IL18 | IGIF | IL-1g | Interleukin-18 | IFN-gamma-inducing factor | IL-1 gamma | IL-18 | Iboctadekin | IL1F4 | Interleukin-1 gamma
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived rat IL-18 recombinant protein (Position: H37-S194). Rat IL-18 shares 64% and 91% amino acid (aa) sequences identity with human and mouse IL-18, respectively.
Epitope
aa37-194
Applications
  • Western blot
  • ELISA
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL18
Q14116 NM_001562 NP_001553.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/26/2024