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Anti-IL17A Antibody LS-C154149

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl 1 mg/ml $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL17A Antibodies

Anti-IL17A Antibody IHC-plus™ LS-B7397
Rabbit Polyclonal (IgG) to Human IL17A
Human
IHC - Paraffin, Western blot, ELISA, Neutralization
Unconjugated
Immunohistochemistry Image
Anti-IL17A Antibody (Biotin) LS-C154151
Rabbit Polyclonal (IgG) to Rat IL17A
Rat, Mouse
IHC, Western blot, ELISA
Biotin Conjugated
Western blot Image
Anti-IL17A Antibody (aa1-75, clone 4K5F6) IHC-plus™ LS-B8323
Mouse Monoclonal [clone 4K5F6] (IgG2b,k) to Human IL17A
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Rat IL17A
Rat, Mouse
Western blot, ELISA
Unconjugated

Details

Rat IL17A
Rabbit
Rat, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Purified, heated to 56°C for 30 minutes
Unmodified

Applications

  • Western blot (1:1000)
  • ELISA

Specificity and Use

IL17A antibody was raised against this purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant rat IL17-A protein.
This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant rat IL17-A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. The recombinant immunogen is a non-glycosylated homodimer joined by disulfide bonds having a molecular mass of 30.0 kD. By western blot from a reducing gel expect a band approximately 15.0 kD in size corresponding to rat IL17-A protein in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About IL17A

Q16552 NM_002190 NP_002181.1

IL17A Antibody, CTLA-8 Antibody, CTLA8 Antibody, Interleukin-17A Antibody, Interleukin 17A Antibody, IL-17 Antibody, IL-17A Antibody, IL17 Antibody

IL17A is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Western blot

Western blot
Anti-IL-17A Antibody - Western Blot. Western blot of anti-IL-17A antibody shows detection of mouse recombinant IL-17A protein (arrowhead, lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1000 dilution. The membrane was washed and reacted with a 1:20000 dilution of DyLight 649 conjugated Gt-a-Rabbit IgG (p/n. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left. Other detection systems will yield similar results.

Western blot

Western blot
Anti-IL-17A Antibody - Western Blot. Western blot of anti-IL-17A antibody shows detection of rat recombinant IL-17A protein (lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1000 dilution. The membrane was washed and reacted with a 1:20000 dilution of DyLight 649 conjugated Gt-a-Rabbit IgG (. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left. Other detection systems will yield similar results.

Requested From: United States
Date Requested: 12/8/2016

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