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Catalog Number Size Price
LS-C154082-100 100 µg (1 mg/ml) $567 
IL17A Antibody - Anti-Human IL17-A Antibody - Western Blot. Western blot of anti-Human IL17-A antibody shows detection of a band ~17 kD in size corresponding to recombinant human IL17-A (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG ( secondary antibody diluted 1:20000 in blocking buffer (p/n MB-070). Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).

Polyclonal Rabbit anti‑Human IL17A Antibody (Lyophilized, IHC, WB) LS‑C154082

Polyclonal Rabbit anti‑Human IL17A Antibody (Lyophilized, IHC, WB) LS‑C154082

Antibody:
IL17A Rabbit anti-Human Polyclonal (Lyophilized) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Lyophilized
Price
Catalog Number
$567
LS-C154082-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IL17A Rabbit anti-Human Polyclonal (Lyophilized) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Lyophilized

Specifications

Description
IL17A antibody LS-C154082 is an unconjugated rabbit polyclonal antibody to human IL17A. Validated for ELISA, IHC and WB.
Target
Human IL17A
Synonyms
IL17A | CTLA-8 | CTLA8 | Interleukin-17A | Interleukin 17A | IL-17 | IL-17A | IL17
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Lyophilized
Immunogen
IL-17A Antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL17-A protein.
Specificity
This antibody will recognize both native and recombinant human IL-17A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended. Cross-reactivity with IL-17 from other sources has not been determined.
Applications
  • IHC (1:100)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:1000 - 1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 30.3 kD in size corresponding to human IL17-A protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL17A
Q16552 NM_002190 NP_002181.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/26/2024