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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL17A Antibody (clone 20B4.G10.F5) LS‑C153842
IL17A antibody LS-C153842 is an unconjugated rat monoclonal antibody to mouse IL17A. Validated for ELISA and WB.
Catalog
Size
Price
LS-C153842-100
100 µg
Unavailable

Popular IL17A Products

Untreated (A) and PMA/ionomycin -stimulated (B) human PBMCs were stained using PE-conjugated mouse anti-human CD3 antibody and APC-conjugated antibody LS-C37030 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry
Western Blot (reducing) of IL-17 antibody LS-C104427.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Western blot, ELISA, Neutralization
Anti-IL-17 antibody IHC of human t lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7397 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA, Neutralization
Biotin Rabbit Anti-Rat IL-17A Antibody - Western Blot. Western Blot showing detection of Rat IL-17A. 50 ng of Rat IL-17A (Lane 1) was run on a 4-20% gel and transferred to 0.45 micron nitrocellulose. After blocking with 5% Blotto (B501-0500) 30 min at 20°C, Anti-Rat IL-17A (RABBIT) Antibody Biotin Conjugated LS-C154151 secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent Western Blot (p/n MB-070). HRP Streptavidin (p/n S000-03) was used at 1:40000 in MB-070 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc 4000 MP. Arrow indicates correct 15 kD molecular weight position expected for Rat IL-17A. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rat, Mouse
Applications: IHC, Western blot, ELISA
Anti-IL-17 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8323 dilution 5-10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

Product Description

IL17A antibody LS-C153842 is an unconjugated rat monoclonal antibody to mouse IL17A. Validated for ELISA and WB.
About IL17A
IL17A is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. Q16552 NM_002190 NP_002181.1

IL17A Antibody, CTLA-8 Antibody, CTLA8 Antibody, Interleukin-17A Antibody, Interleukin 17A Antibody, IL-17 Antibody, IL-17A Antibody, IL17 Antibody


Specifications

Target
Mouse IL17A
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal [20B4.G10.F5]
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • ELISA
Immunogen
IL17A antibody was raised against this Protein A purified monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse IL-17A protein (produced in E. coli) followed by hybridoma development.
Specificity
This antibody is specific for mouse IL-17A protein. Cross-reactivity with IL-17A from other sources has not been determined. Immunology Research.
Usage
This purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 23 kD in size corresponding to the mature mouse IL-17A protein, a non-glycosylated polypeptide chain consisting of 207 amino acids, by western blotting in appropriate cell lysate or extract.
Presentation
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

monoclonal anti IL17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 g/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 g/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 g/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (LS-C153842) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for positive and negative controls.
monoclonal anti IL17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 g/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 g/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 g/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (LS-C153842) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for positive and negative controls.

Flow Cytometry

monoclonal anti IL-17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 g/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 g/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 g/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (LS-C153842) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for monoclonal anti mouse IL-17A antibody (LS-C153842)
monoclonal anti IL-17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 g/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 g/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 g/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (LS-C153842) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for monoclonal anti mouse IL-17A antibody (LS-C153842)

Western blot

monoclonal anti IL17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 g/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 g/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 g/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (LS-C153842) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for positive and negative controls.
monoclonal anti IL17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 g/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 g/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 g/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (LS-C153842) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for positive and negative controls.

Flow Cytometry

monoclonal anti IL-17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 g/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 g/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 g/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (LS-C153842) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for monoclonal anti mouse IL-17A antibody (LS-C153842)
monoclonal anti IL-17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 g/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 g/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 g/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (LS-C153842) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for monoclonal anti mouse IL-17A antibody (LS-C153842)

Western blot

monoclonal anti IL17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 g/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 g/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 g/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (LS-C153842) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for positive and negative controls.
monoclonal anti IL17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 g/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 g/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 g/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (LS-C153842) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for positive and negative controls.

Flow Cytometry

monoclonal anti IL-17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 g/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 g/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 g/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (LS-C153842) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for monoclonal anti mouse IL-17A antibody (LS-C153842)
monoclonal anti IL-17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 g/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 g/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 g/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (LS-C153842) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for monoclonal anti mouse IL-17A antibody (LS-C153842)

Western blot

monoclonal anti IL17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 g/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 g/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 g/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (LS-C153842) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for positive and negative controls.
monoclonal anti IL17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 g/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 g/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 g/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (LS-C153842) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for positive and negative controls.

Flow Cytometry

monoclonal anti IL-17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 g/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 g/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 g/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (LS-C153842) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for monoclonal anti mouse IL-17A antibody (LS-C153842)
monoclonal anti IL-17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 g/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 g/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 g/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (LS-C153842) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for monoclonal anti mouse IL-17A antibody (LS-C153842)


Requested From: 
Date Requested: 6/25/2018

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