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Antibodies > IL17A > LS-C153842
 
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Catalog Number Size Price
LS-C153842-100 100 µg (2.94 mg/ml) $574 
IL17A Antibody - monoclonal anti IL17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 g/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 g/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 g/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (Anti-Mouse IL-17A (RAT) Monoclonal Antibody - 210-501-B32) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for positive and negative controls.
IL17A Antibody - monoclonal anti IL-17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 g/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 g/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 g/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (Anti-Mouse IL-17A (RAT) Monoclonal Antibody - 210-501-B32) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for monoclonal anti mouse IL-17A antibody
IL17A Antibody - monoclonal anti IL17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 g/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 g/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 g/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (Anti-Mouse IL-17A (RAT) Monoclonal Antibody - 210-501-B32) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for positive and negative controls.
IL17A Antibody - monoclonal anti IL-17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 g/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 g/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 g/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (Anti-Mouse IL-17A (RAT) Monoclonal Antibody - 210-501-B32) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for monoclonal anti mouse IL-17A antibody
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Monoclonal Rat anti‑Mouse IL17A Antibody (clone 20B4.G10.F5, WB) LS‑C153842

Monoclonal Rat anti‑Mouse IL17A Antibody (clone 20B4.G10.F5, WB) LS‑C153842

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Antibody:
IL17A Rat anti-Mouse Monoclonal (20B4.G10.F5) Antibody
Application:
WB, Flo, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$574
LS-C153842-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
IL17A Rat anti-Mouse Monoclonal (20B4.G10.F5) Antibody
Application:
WB, Flo, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
IL17A antibody LS-C153842 is an unconjugated rat monoclonal antibody to mouse IL17A. Validated for ELISA, Flow and WB.
Target
Mouse IL17A
Synonyms
IL17A | CTLA-8 | CTLA8 | Interleukin-17A | Interleukin 17A | IL-17 | IL-17A | IL17
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
20B4.G10.F5
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Unmodified
Immunogen
This Mouse IL-17A monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse IL-17A protein (produced in E.coli) followed by hybridoma development.
Specificity
This antibody is specific for mouse IL-17A protein. Cross-reactivity with IL-17A from other sources has not been determined.
Applications
  • Western blot (1:1000)
  • Flow Cytometry (1:500)
  • ELISA (1:10000)
Usage
This purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 23 kD in size corresponding to the mature mouse IL-17A protein, a non-glycosylated polypeptide chain consisting of 207 amino acids, by western blotting in appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL17A
Q16552 NM_002190 NP_002181.1

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IL17A Antibody - Biotin Rabbit Anti-Rat IL-17A Antibody - Western Blot. Western Blot showing detection of Rat IL-17A. 50 ng of Rat IL-17A (Lane 1) was run on a 4-20% gel and transferred to 0.45 micron nitrocellulose. After blocking with 5% Blotto (B501-0500) 30 min at 20?, Anti-Rat IL-17A (RABBIT) Antibody Biotin Conjugated Anti-Rat IL-17A (RABBIT) Antibody Biotin Conjugated - 212-406-B32 secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent Western Blot (p/n MB-070). HRP Streptavidin (p/n S000-03) was used at 1:40000 in MB-070 for 30 min at 20? and imaged using the Bio-Rad VersaDoc 4000 MP. Arrow indicates correct 15 kD molecular weight position expected for Rat IL-17A. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Applications: IHC, Western blot, ELISA
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
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