(tested or 100% immunogen sequence identity)
- Western blot (1 - 5 µg/ml)
- Flow Cytometry
- Sandwich ELISA
IL13 antibody was raised against cOS-expressed recombinant human IL-13.
Recognizes human Interleukin-13 (IL-13).
Suitable for use in ELISA, Immunofluorescence, Flow Cytometry, Western Blot and neutralization. Immunofluorescence/Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-13-positive cells within mixed cell populations. Recombinant human IL-13, or Rat IgG1 should be used as a specificity control. Western Blot: 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-13 under reducing conditions.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
For research use only.