Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 4E9] (IgG2b,k) to Human IL-33
Western blot, ELISA
Human (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [4E9]
Protein G purified
Western blot (1:500 - 1:2000)
IHC - Paraffin
Specificity and Use
IL-33 antibody was raised against recombinant human IL-33 (112-270aa) purified from E. coli.
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since applications vary, each investigator should titrate the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500-1:2000.
PBS, pH 7.4, 0.1% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells.
The Cell lysates of Jurkat (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IL-33 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Requested From: United States
Date Requested: 2/25/2017