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Anti-IL-1B / IL-1 Beta Antibody LS-C104781

Note: This antibody replaces LS-C7703, LS-C53695, LS-C42280
Catalog Size Price
LS-C104781-50 50 µg Unavailable
LS-C104781-100 100 µg Unavailable

Most Popular IL-1B / IL-1 Beta Antibodies

Anti-IL-1B / IL-1 Beta Antibody (clone DF8, Azide-free) LS-C7686
Mouse Monoclonal [clone DF8] (IgG1) to Pig IL-1B / IL-1 Beta
Pig
Western blot
Unconjugated, Azide-free
Anti-IL-1B / IL-1 Beta Antibody (N-Terminus) LS-C18827
Rabbit Polyclonal to Human IL-1B / IL-1 Beta
Human, Dog, Primate
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA, Radioimmunoassay
Unconjugated
Anti-Human IL-1B Antibody - Western Blot. IL-1B was used at a 1:200 dilution incubated 1 h at room temperature to detect dog IL-1B by Western blot. Lane 1 and 2 were loaded with 2.5 ug and 500 ng of dog IL-1B respectively. The molecular weight of the detected band is estimated by comparison to molecular weight markers (not shown). Detection occurred using a 1:3000 dilution of IRDye800 conjugated Donkey anti-Rabbit IgG (code # for 1h at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Anti-IL-1B / IL-1 Beta Antibody (clone AS10) LS-C26495
Mouse Monoclonal [clone AS10] (IgG1)
Human
IHC, Immunoprecipitation, ELISA, Neutralization
Unconjugated
Anti-IL-1B / IL-1 Beta Antibody LS-C42757
Rabbit Polyclonal (IgG) to Sheep IL-1B / IL-1 Beta
Sheep
Western blot, ELISA
Unconjugated
Anti-IL-1B / IL-1 Beta Antibody (clone 3E1) LS-C174781
Mouse Monoclonal [clone 3E1] (IgG1) to Human IL-1B / IL-1 Beta
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IL1B mouse monoclonal antibody.

100% Guaranteed Publications (1)
Rabbit Polyclonal to Rat IL-1B / IL-1 Beta
Rat
IHC, Western blot, ELISA, Neutralization (applications tested for the base form of this product only)
Unconjugated

Details

Rat IL-1B / IL-1 Beta
Rabbit
Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin.
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • Western blot
  • ELISA (0.5 - 2 µg/ml)
  • Neutralization (0.09 - 0.14 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

E.coli derived recombinant Rat IL-1beta.
Rat IL-1beta
Immunohistochemistry: This antibody stained colchicine injected rat brain (including the ventricles and the CA3 region of the hippocampus) tissue. The primary antibody was incubated at 0.25 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Rat IL-1beta (0.20 ng/ml), a concentration of 0.09-0.14 ug/ml of this antibody is required. ELISA: To detect Rat IL-1beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-1beta (LS-C104780) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-1beta. Western Blot: To detect Rat IL-1beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-1beta is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

Publications (1)

1
Effect of Roux-en-Y Gastric Bypass on the NLRP3 Inflammasome in Adipose Tissue from Obese Rats. Mocanu AO, Mulya A, Huang H, Dan O, Shimizu H, Batayyah E, Brethauer SA, Dinischiotu A, Kirwan JP. PloS one. 2015 10:e0139764. (IHC; Rat) [Full Text Article] [PubMed:26437377] [PMC:PMC4593548]

About IL-1B / IL-1 Beta

P01584 NM_000576 NP_000567.1

IL1B Antibody, IL-1B Antibody, IL-1 beta Antibody, IL1-BETA Antibody, Preinterleukin 1 beta Antibody, Pro-interleukin-1-beta Antibody, Catabolin Antibody, IL-1 Antibody, IL1F2 Antibody, Interleukin 1, beta Antibody, Interleukin-1 beta Antibody

IL-1 Beta is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis.

Immunohistochemistry

IHC of IL1B / IL-1 Beta antibody LS-C104781.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC of IL1B / IL-1 Beta antibody LS-C104781. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

IHC of IL1B / IL-1 Beta antibody LS-C104781.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC of IL1B / IL-1 Beta antibody LS-C104781. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of IL1B / IL-1 Beta antibody LS-C104781.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IL1B / IL-1 Beta antibody LS-C104781. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IL1B / IL-1 Beta antibody LS-C104781.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IL1B / IL-1 Beta antibody LS-C104781. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL1B / IL-1 Beta antibody LS-C104781.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IL1B / IL-1 Beta antibody LS-C104781. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 4/21/2018

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