LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
IL‑1B / IL‑1 Beta Antibody LS‑C104781
Note: This antibody replaces LS-C7703, LS-C53695, LS-C42280
IL-1 Beta antibody LS-C104781 is an unconjugated rabbit polyclonal antibody to rat IL-1 Beta (IL-1B). Validated for ELISA, Neut and WB. Cited in 1 publication.
Catalog
Size
Price
LS-C104781-50
50 µg
Unavailable
LS-C104781-100
100 µg
Unavailable

Popular IL-1B / IL-1 Beta Products

Anti-Human IL-1B Antibody - Western Blot. IL-1B was used at a 1:200 dilution incubated 1 h at room temperature to detect dog IL-1B by Western blot. Lane 1 and 2 were loaded with 2.5 ug and 500 ng of dog IL-1B respectively. The molecular weight of the detected band is estimated by comparison to molecular weight markers (not shown). Detection occurred using a 1:3000 dilution of IRDye800 conjugated Donkey anti-Rabbit IgG (code # for 1h at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Species: Human, Dog, Primate
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA, Radioimmunoassay
Antibody
Species: Human
Applications: IHC, Immunoprecipitation, ELISA, Neutralization
Antibody
Species: Sheep
Applications: Western blot, ELISA
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IL1B mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

Product Description

IL-1 Beta antibody LS-C104781 is an unconjugated rabbit polyclonal antibody to rat IL-1 Beta (IL-1B). Validated for ELISA, Neut and WB. Cited in 1 publication.
About IL-1B / IL-1 Beta
IL-1 Beta is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. P01584 NM_000576 NP_000567.1

IL1B Antibody, IL-1B Antibody, IL-1 beta Antibody, IL1-BETA Antibody, Preinterleukin 1 beta Antibody, Pro-interleukin-1-beta Antibody, Catabolin Antibody, IL-1 Antibody, IL1F2 Antibody, Interleukin 1, beta Antibody, Interleukin-1 beta Antibody


Specifications

Target
Rat IL-1B / IL-1 Beta
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • ELISA (0.5 - 2 µg/ml)
  • Neutralization (0.09 - 0.14 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
E.coli derived recombinant Rat IL-1beta.
Specificity
Rat IL-1beta
Usage
Immunohistochemistry: This antibody stained colchicine injected rat brain (including the ventricles and the CA3 region of the hippocampus) tissue. The primary antibody was incubated at 0.25 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Rat IL-1beta (0.20 ng/ml), a concentration of 0.09-0.14 ug/ml of this antibody is required. ELISA: To detect Rat IL-1beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-1beta (LS-C104780) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-1beta. Western Blot: To detect Rat IL-1beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-1beta is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (1)

1
Effect of Roux-en-Y Gastric Bypass on the NLRP3 Inflammasome in Adipose Tissue from Obese Rats. Mocanu AO, Mulya A, Huang H, Dan O, Shimizu H, Batayyah E, Brethauer SA, Dinischiotu A, Kirwan JP. PloS one. 2015 10:e0139764. (IHC; Rat) [Full Text Article] [PubMed:26437377] [PMC:PMC4593548]

Customer Reviews (0)


Images


Western blot

Western Blot (reducing) of IL1B / IL-1 Beta antibody LS-C104781.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IL1B / IL-1 Beta antibody LS-C104781. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IL1B / IL-1 Beta antibody LS-C104781.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IL1B / IL-1 Beta antibody LS-C104781. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL1B / IL-1 Beta antibody LS-C104781.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IL1B / IL-1 Beta antibody LS-C104781. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of IL1B / IL-1 Beta antibody LS-C104781.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IL1B / IL-1 Beta antibody LS-C104781. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IL1B / IL-1 Beta antibody LS-C104781.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IL1B / IL-1 Beta antibody LS-C104781. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL1B / IL-1 Beta antibody LS-C104781.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IL1B / IL-1 Beta antibody LS-C104781. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of IL1B / IL-1 Beta antibody LS-C104781.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IL1B / IL-1 Beta antibody LS-C104781. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IL1B / IL-1 Beta antibody LS-C104781.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IL1B / IL-1 Beta antibody LS-C104781. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL1B / IL-1 Beta antibody LS-C104781.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IL1B / IL-1 Beta antibody LS-C104781. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of IL1B / IL-1 Beta antibody LS-C104781.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IL1B / IL-1 Beta antibody LS-C104781. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IL1B / IL-1 Beta antibody LS-C104781.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IL1B / IL-1 Beta antibody LS-C104781. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL1B / IL-1 Beta antibody LS-C104781.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IL1B / IL-1 Beta antibody LS-C104781. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/23/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number