Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Specificity and Use
His-tagged, full-length human IKKg/IKK3.
Recognizes IKKg/IKK3, Mr 48kD. IKKg/IKK3 is also known as NEMO, FIP-3 and IKKAP-1. Species cross-reactivity: Human and mouse.
Suitable for use in ELISA and Western Blot. Western Blot Analysis: 0.5-2 ug/ml detects IKKg/IKK3 in RIPA lysates from Jurkat and 3T3 cells. Jurkat lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-IKKg/IKK3 (0.5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
PBS, pH 7.4, 0.01% sodium azide, 40% glycerol.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways.