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Anti-IKBKB / IKK2 / IKK Beta Antibody (C-Terminus) IHC-plus™ LS-B1362

Note: This antibody replaces LS-C306, LS-C42828, LS-C57039, LS-C15907, LS-C121236, LS-C15897

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

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Most Popular IKBKB / IKK2 / IKK Beta Antibodies

Anti-IKBKB / IKK2 / IKK Beta Antibody (C-Terminus) IHC-plus™ LS-B715
Rabbit Polyclonal to Human IKBKB / IKK2 / IKK Beta
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-IKBKB / IKK2 / IKK Beta Antibody (phospho-Ser697) LS-C159908
Rabbit Polyclonal to Human IKBKB / IKK2 / IKK Beta
Human
Dot Blot
Unconjugated
Dot Blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human IKBKB / IKK2 / IKK Beta
Human
IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human IKBKB / IKK2 / IKK Beta
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Western blot (1:500)

Specificity and Use

IKBKB / IKK2 / IKK Beta antibody was raised against a peptide corresponding to amino acids near the carboxy-terminus of human IKK beta (Genbank accession NoO14920), which differs from corresponding murine sequence by one amino acid.
LS-E29892 - Liquid - 50 µg - $145.00
C-Terminus
peptide corresponding to amino acids near the carboxy-terminus of human IKKb (Genbank accession NoO14920), which differs from corresponding murine sequence by one amino acid
Immunohistochemistry: LS-B1362 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1362 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About IKBKB / IKK2 / IKK Beta

O14920 AF080158 AAD08997.1

IKBKB Antibody, Ikk 2 Antibody, IKK-B Antibody, IKK2 Antibody, IKKbeta Antibody, NFKBIKB Antibody, I-kappa-B kinase 2 Antibody, IKKB Antibody, I-kappa-B-kinase beta Antibody, Ikb kinase-beta Antibody, IKK Beta Antibody, IKK-beta Antibody

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome.

Immunohistochemistry

Immunohistochemistry
Anti-IKBKB / IKK Beta antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1362 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry
Immunocytochemistry staining of HeLa cells using IKK beta antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of IKK beta in HeLa cells with IKK beta antibody at 10 ug/ml.

Western blot

Western blot
Western blot of IKK beta in Jurkat whole cell lysate with IKK beta antibody (C3) at 1:500 dilution.

Requested From: United States
Date Requested: 12/2/2016

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