LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
IKBKB / IKK2 / IKK Beta Antibody (C‑Terminus) IHC‑plus™ LS‑B1362
Note: This antibody replaces LS-C306, LS-C42828, LS-C57039, LS-C15907, LS-C121236, LS-C15897
IKK Beta antibody LS-B1362 is an unconjugated rabbit polyclonal antibody to human IKK Beta (IKBKB / IKK2). Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B1362-50
50 µg (1 mg/ml)
Unavailable

Popular IKBKB / IKK2 / IKK Beta Products

Antibody
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA
Anti-IKBKB / IKK Beta antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B715 dilution 1:500.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Western blot Image
Species: Human, Mouse, Rat
Applications: Western blot, Immunoprecipitation, ELISA

Product Description

IKK Beta antibody LS-B1362 is an unconjugated rabbit polyclonal antibody to human IKK Beta (IKBKB / IKK2). Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About IKBKB / IKK2 / IKK Beta
Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. O14920 AF080158 AAD08997.1

IKBKB Antibody, Ikk 2 Antibody, IKK-B Antibody, IKK2 Antibody, IKKbeta Antibody, NFKBIKB Antibody, I-kappa-B kinase 2 Antibody, IKKB Antibody, I-kappa-B-kinase beta Antibody, Ikb kinase-beta Antibody, IKK Beta Antibody, IKK-beta Antibody


Specifications

Target
Human IKBKB / IKK2 / IKK Beta
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Western blot (1:500)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E29892 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B1362. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
This polyclonal antibody has no cross response to IKKa or IKKy.
Usage
Immunohistochemistry: LS-B1362 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1362 was determined to be 5 ug/ml.
Presentation
PBS, 0.02% sodium azide.
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Immunohistochemistry

Anti-IKBKB / IKK Beta antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1362 concentration 5 ug/ml.
Anti-IKBKB / IKK Beta antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1362 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry staining of HeLa cells using IKK beta antibody at 10 ug/ml.
Immunocytochemistry staining of HeLa cells using IKK beta antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of IKK beta in HeLa cells with IKK beta antibody at 10 ug/ml.
Immunofluorescence of IKK beta in HeLa cells with IKK beta antibody at 10 ug/ml.

Western blot

Western blot of IKK beta in Jurkat whole cell lysate with IKK beta antibody (C3) at 1:500 dilution.
Western blot of IKK beta in Jurkat whole cell lysate with IKK beta antibody (C3) at 1:500 dilution.

Immunohistochemistry

Anti-IKBKB / IKK Beta antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1362 concentration 5 ug/ml.
Anti-IKBKB / IKK Beta antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1362 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry staining of HeLa cells using IKK beta antibody at 10 ug/ml.
Immunocytochemistry staining of HeLa cells using IKK beta antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of IKK beta in HeLa cells with IKK beta antibody at 10 ug/ml.
Immunofluorescence of IKK beta in HeLa cells with IKK beta antibody at 10 ug/ml.

Western blot

Western blot of IKK beta in Jurkat whole cell lysate with IKK beta antibody (C3) at 1:500 dilution.
Western blot of IKK beta in Jurkat whole cell lysate with IKK beta antibody (C3) at 1:500 dilution.

Immunohistochemistry

Anti-IKBKB / IKK Beta antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1362 concentration 5 ug/ml.
Anti-IKBKB / IKK Beta antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1362 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry staining of HeLa cells using IKK beta antibody at 10 ug/ml.
Immunocytochemistry staining of HeLa cells using IKK beta antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of IKK beta in HeLa cells with IKK beta antibody at 10 ug/ml.
Immunofluorescence of IKK beta in HeLa cells with IKK beta antibody at 10 ug/ml.

Western blot

Western blot of IKK beta in Jurkat whole cell lysate with IKK beta antibody (C3) at 1:500 dilution.
Western blot of IKK beta in Jurkat whole cell lysate with IKK beta antibody (C3) at 1:500 dilution.

Immunohistochemistry

Anti-IKBKB / IKK Beta antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1362 concentration 5 ug/ml.
Anti-IKBKB / IKK Beta antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1362 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry staining of HeLa cells using IKK beta antibody at 10 ug/ml.
Immunocytochemistry staining of HeLa cells using IKK beta antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of IKK beta in HeLa cells with IKK beta antibody at 10 ug/ml.
Immunofluorescence of IKK beta in HeLa cells with IKK beta antibody at 10 ug/ml.

Western blot

Western blot of IKK beta in Jurkat whole cell lysate with IKK beta antibody (C3) at 1:500 dilution.
Western blot of IKK beta in Jurkat whole cell lysate with IKK beta antibody (C3) at 1:500 dilution.


Requested From: 
Date Requested: 6/24/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number