(tested or 100% immunogen sequence identity)
IGSF5 / JAM4 antibody was raised against nS0-derived rmJAM4 (aa16-140).
Selected for use as a detection antibody in mouse JAM4 Western Blots.
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect recombinant mouse JAM4. The detection limit for rmJAM4 is approximately 5 ng/lane under non-reducing and reducing conditions. In this format, this antibody shows less than 1% cross-reactivity with rmJAM-A, rmJAM-B, and rmJAM-C. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.