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Anti-IGF2BP2 Antibody (clone 1F9) LS-C115283

Catalog Size Price
LS-C115283-100 100 µl Unavailable

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100% Guaranteed
Mouse Monoclonal [clone 1F9] (IgG1) to Human IGF2BP2
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human IGF2BP2
Mouse
IgG1 Monoclonal [1F9]
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:1000 - 1:2000)
  • Flow Cytometry (1:100)

Specificity and Use

IGF2BP2 antibody was raised against full length human recombinant protein of human IGF2BP2(NP_006539) produced in HEK293T cell
Human IGF2BP2
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About IGF2BP2

Q9Y6M1 NM_006548 NP_006539.3

IGF2BP2 Antibody, IMP2 Antibody, IGF2 mRNA-binding protein 2 Antibody, p62 Antibody, VICKZ2 Antibody, IGF-II mRNA-binding protein 2 Antibody, IMP-2 Antibody, VICKZ family member 2 Antibody

RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation (By similarity). Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific.

Immunohistochemistry

Immunohistochemistry
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IGF2BP2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-IGF2BP2 mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Immunofluorescence
Anti-IGF2BP2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IGF2BP2.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2.

Western blot

Western blot
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IGF2BP2 monoclonal antibody.

Flow Cytometry

Flow Cytometry
Flow cytometric Analysis of Jurkat cells, using anti-IGF2BP2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IGF2BP2 antibody, and then analyzed by flow cytometry.

Requested From: 
Date Requested: 10/18/2017

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