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Catalog Number Size Price
LS-B8345-50 50 µg (0.5 mg/ml) $460 
IGF1 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
IGF1 Antibody - IGF1 Antibody Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
IGF1 Antibody - IGF1 antibody (0.5µg/ml) staining of Human Uterus lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
IGF1 Antibody - IGF1 antibody (0.5 ug/ml) staining of Human Uterus lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
IGF1 Antibody - IGF1 Antibody Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
IGF1 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
IGF1 Antibody - IGF1 Antibody Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
IGF1 Antibody - IGF1 antibody (0.5µg/ml) staining of Human Uterus lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
IGF1 Antibody - IGF1 antibody (0.5 ug/ml) staining of Human Uterus lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
IGF1 Antibody - IGF1 Antibody Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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IHC‑plus™ Polyclonal Goat anti‑Human IGF1 Antibody (aa100‑109, IHC, IF, WB) LS‑B8345

IHC‑plus™ Polyclonal Goat anti‑Human IGF1 Antibody (aa100‑109, IHC, IF, WB) LS‑B8345

Note: This antibody replaces LS-C139648
Antibody:
IGF1 Goat anti-Human Polyclonal (aa100-109) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B8345-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IGF1 Goat anti-Human Polyclonal (aa100-109) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Format:
Unconjugated, Unmodified

Specifications

Description
IGF1 antibody LS-B8345 is an unconjugated goat polyclonal antibody to IGF1 (aa100-109) from human. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human IGF1
Synonyms
IGF1 | IGF-IA | IGF-IB | IGFI | IBP1 | IGF-I | Insulin-like growth factor 1 | Insulin-like growth factor I | Insulin-like growth factor IA | Insulin-like growth factor IB | Mechano growth factor | Somatomedin-C | IGF1A | MGF
Host
Goat
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-RSVRAQRHTD, from the internal region of the protein sequence according to NP_001104753.1NP_001104754.1NP_001104755.1NP_000609.1.
Epitope
aa100-109
Specificity
Human IGF1. This antibody is expected to recognize all reported isoforms (NP_001104753.1; NP_001104754.1; NP_001104755.1; NP_000609.1).
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (0.5 - 2 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 15kD band observed in Human Uterus lysates (calculated MW of 15.2kD according to NP_001104754.1). Recommended concentration 0.5-2 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IGF1
P05019 NM_000618 NP_000609.1

Validation

IGF1 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ IGF1 Antibody (aa100-109) for IHC, IF/Immunofluorescence, WB/Western LS-B8345 has an LSBio Rating of
Laboratory Validation Score (4)

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Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/26/2024