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Catalog Number Size Price
LS-C153817-100 100 µg (1 mg/ml) $567 
IFN Gamma / Interferon Gamma Antibody - Western blot of protein-A purified anti-bovine IFN gamma antibody shows detection of recombinant bovine IFN gamma at 16.9 kDa, raised in yeast. Primary antibody was diluted to 1g/mL. 3% BSA from BSA-30 (Bovine Serum Albumin Solution) was used for blocking. Secondary antibody 611-131-122 (Goat anti-Rabbit IgG IRDye 800) was used at 1:20000.

Polyclonal Rabbit anti‑Bovine IFN Gamma / Interferon Gamma Antibody (Lyophilized, WB) LS‑C153817

Polyclonal Rabbit anti‑Bovine IFN Gamma / Interferon Gamma Antibody (Lyophilized, WB) LS‑C153817

Antibody:
IFN Gamma / Interferon Gamma Rabbit anti-Bovine Polyclonal (Lyophilized) Antibody
Application:
WB, ELISA
Reactivity:
Bovine
Format:
Unconjugated, Lyophilized
Price
Catalog Number
$567
LS-C153817-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IFN Gamma / Interferon Gamma Rabbit anti-Bovine Polyclonal (Lyophilized) Antibody
Application:
WB, ELISA
Reactivity:
Bovine
Format:
Unconjugated, Lyophilized

Specifications

Description
Interferon Gamma antibody LS-C153817 is an unconjugated rabbit polyclonal antibody to bovine Interferon Gamma (IFN Gamma). Validated for ELISA and WB.
Target
Bovine IFN Gamma / Interferon Gamma
Synonyms
IFNG | IFG | Immune interferon | IFI | Interferon gamma | IFN-gamma | Interferon, gamma | IFNY
Host
Rabbit
Reactivity
Bovine (tested or 100% immunogen sequence identity)
Predicted
Goat, Sheep, Zebu, Bison, Camel, Giraffe, Red deer, Sika deer (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Lyophilized
Immunogen
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast corresponding to the 143 amino acids of the mature Bovine IFN Gamma protein.
Specificity
This antibody is specific for bovine IFN gamma protein. A BLAST analysis was used to suggest cross-reactivity with IFN gamma from bovine based on 100% homology; cross-reactivity to yak, bison, zebu, buffalo, goat, sheep, nilgai, giraffe, Chinese forest musk deer, sika deer, red deer, Arabian camel, and Bactrian camel based on 91-99% homology with the immunizing sequence. Cross-reactivity with IFN gamma from other sources has not been determined.
Applications
  • Western blot (1:500 - 1:2000)
  • ELISA (1:20000)
Usage
This protein A purified antibody is suitable for ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 16.9 kD in size corresponding to bovine IFN gamma by western blotting in the appropriate cell lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IFN Gamma / Interferon Gamma
P01579 NM_000619 NP_000610.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/26/2024