Human, Mouse (tested or 100% immunogen sequence identity)
Immunofluorescence (1:50 - 1:1000), Western blot (1:2000 - 1:10000), ELISA
Specificity and Use
IDO2 antibody was raised against iDO2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a mixture of human and mouse full length protein corresponding to IDO2.
Anti-IDO2 antibody is directed against IDO2 protein. The rabbit polyclonal antibody was affinity purified against the antigen. Prior to affinity purification the antibody was pre-cleared of any cross reactive antibody to IDO1 (human and mouse). A BLAST analysis was used to suggest cross-reactivity with IDO2 protein from human and mouse 100% based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Anti-IDO2 antibody has been tested for use in ELISA, IF, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 45.4 kD in size corresponding to IDO2 protein by western blotting in the appropriate cell lysate or extract. IHC testing has been negative.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
Immunofluorescence of Anti-IDO2 Antibody. Tissue: human IDO2 expressing HEK293 cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary: IDO2 antibody at 1:50-1:1000 for 1 h at RT. Secondary: Peroxidase rabbit secondary antibody at 1:10000 for 45min at RT. Localization: IDO-2 is located in the cytosol. Staining: IDO-2 as green fluorescent signal with bis-benzimide nuclear counterstain (blue).
Requested From: United States
Date Requested: 4/20/2015