Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse (tested or 100% immunogen sequence identity)
Immunofluorescence (1:50 - 1:1000), Western blot (1:2000 - 1:10000), ELISA
Specificity and Use
IDO2 / INDOL1 antibody was raised against iDO2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a mixture of human and mouse full length protein corresponding to IDO2.
Anti-IDO2 antibody is directed against IDO2 protein. The rabbit polyclonal antibody was affinity purified against the antigen. Prior to affinity purification the antibody was pre-cleared of any cross reactive antibody to IDO1 (human and mouse). A BLAST analysis was used to suggest cross-reactivity with IDO2 protein from human and mouse 100% based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Anti-IDO2 antibody has been tested for use in ELISA, IF, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 45.4 kD in size corresponding to IDO2 protein by western blotting in the appropriate cell lysate or extract. IHC testing has been negative.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
Immunofluorescence of Anti-IDO2 Antibody. Tissue: human IDO2 expressing HEK293 cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary: IDO2 antibody at 1:50-1:1000 for 1 h at RT. Secondary: Peroxidase rabbit secondary antibody at 1:10000 for 45min at RT. Localization: IDO-2 is located in the cytosol. Staining: IDO-2 as green fluorescent signal with bis-benzimide nuclear counterstain (blue).
Requested From: United States
Date Requested: 5/23/2016