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Anti-IDN3 / NIPBL Antibody (aa344-356) LS-C60065

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IDN3 / NIPBL Antibodies

Anti-IDN3 / NIPBL Antibody (clone KT54) IHC-plus™ LS-B7392
Rat Monoclonal [clone KT54] (IgG1) to Human IDN3 / NIPBL
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-IDN3 / NIPBL Antibody IHC-plus™ LS-B7393
Rat Monoclonal (IgG2b) to Human IDN3 / NIPBL
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human IDN3 / NIPBL
Human
Western blot, ELISA
Unconjugated

Details

Human IDN3 / NIPBL
Goat
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:500 - 1:3000)
  • ELISA (1:2000 - 1:10000)

Specificity and Use

IDN3 / NIPBL antibody was raised against synthetic peptide corresponding to amino acids 344-356 of Human IDN3.
aa344-356
human IDN3 protein. A BLAST analysis was used to suggest reactivity with this protein from human, rat, mouse, dog, bovine and orangutan based on 100% homology for the immunogen sequence. Expect reactivity with multiple splice variants of this protein. Cross reactivity with IDN3 homologs from other sources has not been determined
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect bands at 316 kD in size corresponding to IDN3 by western blotting in the appropriate cell lysate or extract. Splice variants for this protein may show molecular weights of 304 kD and 122 kD.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.

About IDN3 / NIPBL

Q6KC79 NM_015384 NP_056199.2

NIPBL Antibody, CDLS1 Antibody, CDLS Antibody, Delangin Antibody, IDN3 Antibody, Nipped-B homolog (Drosophila) Antibody, IDN3-B Antibody, Scc2 Antibody, SCC2 homolog Antibody, Nipped-B-like protein Antibody

IDN3 / NIPBL is the homolog of the Drosophila melanogaster Nipped-B gene product and fungal Scc2-type sister chromatid cohesion proteins. The Drosophila protein facilitates enhancer-promoter communication of remote enhancers and plays a role in developmental regulation. It is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair.

Western blot

Western blot
Anti-IDN3 Antibody - Western Blot. Western blot of Affinity Purified anti-IDN3 antibody shows detection of bands at ~315 kD and ~125 kD corresponding to isoforms of IDN3 (arrow-heads) in mouse heart whole cell tissue extract. Approximately 35 ug of lysate was separated on a 4-8% gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1500. Reaction occurred overnight at 4? followed by washes and reaction with a 1:20000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
 

Requested From: United States
Date Requested: 12/5/2016

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