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Anti-IDH1 / IDH Antibody (aa290-339) IHC-plus™ LS-B3573

Note: This antibody replaces LS-C81929

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IDH1 / IDH Antibodies

Anti-IDH1 / IDH Antibody (aa290-339) IHC-plus™ LS-B3573
Rabbit Polyclonal (IgG) to Human IDH1 / IDH
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-IDH1 / IDH Antibody IHC-plus™ LS-B4836
Rabbit Polyclonal to Human IDH1 / IDH
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-IDH1 / IDH Antibody (C-Terminus) IHC-plus™ LS-B5288
Rabbit Polyclonal to Human IDH1 / IDH
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human IDH1 / IDH
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human IDH1 / IDH
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

IDH1 / IDH antibody was raised against synthetic peptide located between aa290-339 of human IDH1 (Q8LFC0, NP_005887). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Hamster, Dog, Cat, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Beetle (100%); Panda, Armadillo, Rice, Arabidopsis (92%); Drosophila (85%).
LS-E19748 - Lyophilized - 100 µg - $145.00
aa290-339
Human IDH1
Immunohistochemistry: LS-B3573 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3573 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About IDH1 / IDH

O75874 NM_005896 NP_005887.2

IDH1 Antibody, IDCD Antibody, IDP Antibody, IDPC Antibody, PICD Antibody, NADP(+)-specific ICDH Antibody, IDH Antibody, Oxalosuccinate decarboxylase Antibody

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic.

Immunohistochemistry

Immunohistochemistry
Anti-IDH1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3573 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-IDH1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3573 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/5/2016

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