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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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ICAM‑1 / CD54 Antibody (aa70‑119) LS‑C116902
CD54 antibody LS-C116902 is an unconjugated rabbit polyclonal antibody to CD54 (ICAM-1) from human and monkey. Validated for WB.
Catalog
Size
Price
LS-C116902-50
50 µg
Unavailable

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Antibody
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Flow cytometry of ICAM1 / CD54 antibody This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human
Applications: Western blot, Flow Cytometry, ELISA
Flow cytometry of ICAM1 / CD54 antibody This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry, ELISA

Product Description

CD54 antibody LS-C116902 is an unconjugated rabbit polyclonal antibody to CD54 (ICAM-1) from human and monkey. Validated for WB.
About ICAM-1 / CD54
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. P05362 NM_000201 NP_000192.2

ICAM1 Antibody, BB2 Antibody, CD54 antigen Antibody, CD54 Antibody, ICAM-1 Antibody, p3.58 Antibody


Specifications

Target
Human ICAM-1 / CD54
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
ICAM-1 / CD54 antibody was raised against synthetic peptide located between aa70-119 of human ICAM1 (P05362, NP_000192). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%).
Blocking Peptide
LS-E21626 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C116902. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa70-119
Specificity
Human ICAM1 / CD54
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

ICAM1 / CD54 antibody LS-C116902 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ICAM1 / CD54 antibody LS-C116902 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ICAM1 / CD54 antibody LS-C116902 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ICAM1 / CD54 antibody LS-C116902 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ICAM1 / CD54 antibody LS-C116902 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ICAM1 / CD54 antibody LS-C116902 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ICAM1 / CD54 antibody LS-C116902 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ICAM1 / CD54 antibody LS-C116902 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/17/2018

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