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Anti-HUPF2 / UPF2 Antibody (aa51-100) LS-C116856

Ordering

Wt. Vol. Conc. Price
50 µg - - $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HUPF2 / UPF2 Antibodies

Anti-HUPF2 / UPF2 Antibody (aa630-656, AP) LS-C218362
Rabbit Polyclonal (IgG) to Human HUPF2 / UPF2
Human
Western blot, Flow Cytometry, ELISA
AP Conjugated
Anti-HUPF2 / UPF2 Antibody (aa630-656, Azide-free, HRP) LS-C218365
Rabbit Polyclonal (IgG) to Human HUPF2 / UPF2
Human
Western blot, Flow Cytometry, ELISA
HRP Conjugated, Azide-free
Anti-HUPF2 / UPF2 Antibody (aa1-50) IHC-plus™ LS-B14191
Rabbit Polyclonal (IgG) to Human HUPF2 / UPF2
Human, Mouse, Bovine, Dog, Horse, Pig, Rabbit
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human HUPF2 / UPF2
Human, Mouse, Rat, Horse
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human HUPF2 / UPF2
Rabbit
Human, Mouse, Rat, Horse (tested or 100% immunogen sequence identity)
Monkey, Bat, Bovine, Chicken (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

HUPF2 / UPF2 antibody was raised against synthetic peptide located between aa51-100 of human UPF2 (Q9HAU5, NP_056357). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%); Marmoset, Panda, Bovine, Bat, Turkey, Chicken (92%); Galago, Mouse, Rat, Elephant, Horse, Zebra finch, Platypus, Stickleback, Zebrafish (85%); Drosophila (83%).
LS-E14960 - Lyophilized - 100 µg - $145.00
aa51-100
Human UPF2
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About HUPF2 / UPF2

Q9HAU5 NM_015542 NP_056357.1

UPF2 Antibody, DKFZP434D222 Antibody, KIAA1408 Antibody, RENT2 Antibody, Smg-3 Antibody, Yeast Upf2p homolog Antibody, HUPF2 Antibody

Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC). Recruited by UPF3B associated with the EJC core at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF3B stimulates both ATPase and RNA helicase activities of UPF1.

Western blot

Western blot
UPF2 antibody LS-C116856 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/10/2016

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