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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HSPD1 / HSP60 Antibody (clone LK1) IHC‑plus™ LS‑B3414
Note: This antibody replaces LS-C103503
HSP60 antibody LS-B3414 is an unconjugated mouse monoclonal antibody to HSP60 (HSPD1) from human, mouse, rat and other species. Validated for Flow, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B3414-50
50 µg
Unavailable

Popular HSPD1 / HSP60 Products

Antibody
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken, Xenopus, Drosophila
Applications: Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Antibody
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Chicken
Applications: IHC, Western blot, Flow Cytometry
Anti-HSPD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2040 concentration 5 ug/ml.
Species: Human, Monkey, Bovine, Dog
Applications: IHC, IHC - Paraffin, Western blot
PfHsp60, Malarial parasite lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Plasmodium falciparum
Applications: Immunofluorescence, Western blot
 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: Immunoprecipitation

Product Description

HSP60 antibody LS-B3414 is an unconjugated mouse monoclonal antibody to HSP60 (HSPD1) from human, mouse, rat and other species. Validated for Flow, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
About HSPD1 / HSP60
HSPD1 / HSP60 is a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. P10809 NM_002156 NP_002147.2

HSPD1 Antibody, 60 kDa chaperonin Antibody, CPN60 Antibody, Heat shock protein 65 Antibody, Heat shock protein 60 Antibody, HSP-60 Antibody, HSP60 Antibody, HuCHA60 Antibody, HLD4 Antibody, HSP65 Antibody, p60 lymphocyte protein Antibody, SPG13 Antibody, Chaperonin 60 Antibody, GROEL Antibody


Specifications

Target
Human HSPD1 / HSP60
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken, Xenopus, Drosophila (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [LK1]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000)
  • Immunoprecipitation (1:80)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HSPD1 / HSP60 antibody was raised against recombinant human Hsp60 protein.
Usage
Immunohistochemistry: LS-B3414 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3414 was determined to be 10 ug/ml.
Presentation
PBS, pH 7.2, 0.09% azide, 50% glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-HSPD1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3414 concentration 10 ug/ml.
Anti-HSPD1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3414 concentration 10 ug/ml.

Immunohistochemistry

Anti-HSPD1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3414 concentration 10 ug/ml.
Anti-HSPD1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3414 concentration 10 ug/ml.

Immunohistochemistry

Anti-HSPD1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3414 concentration 10 ug/ml.
Anti-HSPD1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3414 concentration 10 ug/ml.

Immunohistochemistry

Anti-HSPD1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3414 concentration 10 ug/ml.
Anti-HSPD1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3414 concentration 10 ug/ml.

Immunohistochemistry

Anti-HSPD1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3414 concentration 10 ug/ml.
Anti-HSPD1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3414 concentration 10 ug/ml.

Immunohistochemistry

Anti-HSPD1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3414 concentration 10 ug/ml.
Anti-HSPD1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3414 concentration 10 ug/ml.

Immunohistochemistry

Anti-HSPD1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3414 concentration 10 ug/ml.
Anti-HSPD1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3414 concentration 10 ug/ml.

Immunohistochemistry

Anti-HSPD1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3414 concentration 10 ug/ml.
Anti-HSPD1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3414 concentration 10 ug/ml.


Requested From: 
Date Requested: 6/20/2018

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