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Catalog Number Size Price
LS-C344091-10 10 µg $318 
LS-C344091-100 100 µg (0.5 mg/ml) $470 
HSPB1 / HSP27 Antibody - HSP27 antibody IHC-frozen: Human Placenta Tissue.
HSPB1 / HSP27 Antibody - HSP27 antibody IHC-paraffin: Human Mammary Cancer Tissue.
HSPB1 / HSP27 Antibody - HSP27 antibody IHC-paraffin: Human Intestinal Cancer Tissue.
HSPB1 / HSP27 Antibody - IF analysis of HSP27 using anti-HSP27 antibody HSP27 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-HSP27 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
HSPB1 / HSP27 Antibody - HSP27 antibody Western blot. All lanes: Anti HSP27 at 0.5 ug/ml. Lane 1: NRK Whole Cell Lysate at 40 ug. Lane 2: HELA Whole Cell Lysate at 40 ug. Lane 3: A549 Whole Cell Lysate at 40 ug. Lane 4: COLO320 Whole Cell Lysate at 40 ug. Lane 5: HEPG2 Whole Cell Lysate at 40 ug. Predicted band size: 23 kD. Observed band size: 23 kD.
HSPB1 / HSP27 Antibody - HSP27 antibody Western blot. All lanes: Anti HSP27 at 0.5 ug/ml. WB: Recombinant Human HSP27 Protein 0.5ng. Predicted band size: 19 kD. Observed band size: 19 kD.
HSPB1 / HSP27 Antibody - Flow Cytometry analysis of A431 cells using anti-HSP27 antibody. Overlay histogram showing A431 cells stained with anti-HSP27 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSP27 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
HSPB1 / HSP27 Antibody - HSP27 antibody IHC-frozen: Human Placenta Tissue.
HSPB1 / HSP27 Antibody - HSP27 antibody IHC-paraffin: Human Mammary Cancer Tissue.
HSPB1 / HSP27 Antibody - HSP27 antibody IHC-paraffin: Human Intestinal Cancer Tissue.
HSPB1 / HSP27 Antibody - IF analysis of HSP27 using anti-HSP27 antibody HSP27 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-HSP27 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
HSPB1 / HSP27 Antibody - HSP27 antibody Western blot. All lanes: Anti HSP27 at 0.5 ug/ml. Lane 1: NRK Whole Cell Lysate at 40 ug. Lane 2: HELA Whole Cell Lysate at 40 ug. Lane 3: A549 Whole Cell Lysate at 40 ug. Lane 4: COLO320 Whole Cell Lysate at 40 ug. Lane 5: HEPG2 Whole Cell Lysate at 40 ug. Predicted band size: 23 kD. Observed band size: 23 kD.
HSPB1 / HSP27 Antibody - HSP27 antibody Western blot. All lanes: Anti HSP27 at 0.5 ug/ml. WB: Recombinant Human HSP27 Protein 0.5ng. Predicted band size: 19 kD. Observed band size: 19 kD.
HSPB1 / HSP27 Antibody - Flow Cytometry analysis of A431 cells using anti-HSP27 antibody. Overlay histogram showing A431 cells stained with anti-HSP27 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSP27 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human HSPB1 / HSP27 Antibody (aa1‑205, IHC, WB) LS‑C344091

Polyclonal Rabbit anti‑Human HSPB1 / HSP27 Antibody (aa1‑205, IHC, WB) LS‑C344091

Antibody:
HSPB1 / HSP27 Rabbit anti-Human Polyclonal (aa1-205) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C344091-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HSPB1 / HSP27 Rabbit anti-Human Polyclonal (aa1-205) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HSP27 antibody LS-C344091 is an unconjugated rabbit polyclonal antibody to human HSP27 (HSPB1) (aa1-205). Validated for IHC and WB.
Target
Human HSPB1 / HSP27
Synonyms
HSPB1 | 28 kDa heat shock protein | Heat shock 27 kDa protein | Heat shock 27kD protein 1 | Heat shock 27kDa protein 1 | HSP27 | HSP28 | HSP 27 | Hsp25 | SRP27 | Stress-responsive protein 27 | CMT2F | Heat shock 27 kd protein | Heat shock protein beta-1 | HMN2B | HS.76067
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human Hsp27 recombinant protein (Position: M1-K205). Human Hsp27 shares 83% amino acid (aa) sequence identity with mouse Hsp27.
Epitope
aa1-205
Specificity
Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle. .
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPB1 / HSP27
P04792 NM_001540 NP_001531.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/26/2024