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Anti-HRH1 / Histamine H1 Receptor Antibody (aa471-484) IHC-plus™ LS-B1745

Note: This antibody replaces LS-C47564, LS-C67886
Catalog Size Price
LS-B1745-50 50 µg (0.5 mg/ml) Unavailable

Most Popular HRH1 / Histamine H1 Receptor Antibodies

Anti-HRH1 / Histamine H1 Receptor Antibody (Extracellular Domain) IHC-plus™ LS-A1167
Rabbit Polyclonal to Human HRH1 / Histamine H1 Receptor
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-HRH1 / Histamine H1 Receptor Antibody (Cytoplasmic Domain) IHC-plus™ LS-A1170
Rabbit Polyclonal to Human HRH1 / Histamine H1 Receptor
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-HRH1 / Histamine H1 Receptor Antibody (C-Terminus) LS-C24224
Rabbit Polyclonal (IgG) to Rat HRH1 / Histamine H1 Receptor
Rat, Mouse, Bovine
Western blot, ELISA
Unconjugated
Anti-HRH1 / Histamine H1 Receptor Antibody (C-Terminus) LS-C24226
Rabbit Polyclonal (IgG) to Rat HRH1 / Histamine H1 Receptor
Rat, Mouse, Bovine
Western blot, ELISA
Unconjugated
Anti-HRH1 / Histamine H1 Receptor Antibody (aa471-484) IHC-plus™ LS-B1745
Goat Polyclonal to Human HRH1 / Histamine H1 Receptor
Human, Bat
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed Publications (1)
Goat Polyclonal to Human HRH1 / Histamine H1 Receptor
Human, Bat
IHC - Paraffin, ELISA
Unconjugated

Details

Human HRH1 / Histamine H1 Receptor
Goat
Human, Bat (tested or 100% immunogen sequence identity)
Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • ELISA (1:32000)

Specificity and Use

Synthetic peptide CNENFKKTFKRILH from the C-terminus of human HRH1 / Histamine H1 Receptor (NP_000852.1; NP_001091681.1; NP_001091682.1; NP_001091683.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Bat, Platypus (100%); Monkey, Mouse, Rat, Dog, Bovine, Hamster, Elephant, Panda, Rabbit, Horse, Pig, Turkey, Chicken (93%); Marmoset, Guinea pig, Xenopus (86%).
LS-E25938 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B1745. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa471-484
Human HRH1 / Histamine H1 Receptor. Variants (NP_000852.1; NP_001091681.1; NP_001091682.1; NP_001091683.1) encode the same protein.
Immunohistochemistry: LS-B1745 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1745 was determined to be 2.5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

Publications (1)

1
Estrogenic regulation of histamine receptor subtype H1 expression in the ventromedial nucleus of the hypothalamus in female rats. Mori H, Matsuda K, Yamawaki M, Kawata M. PloS one. 2014 9:e96232. [Full Text Article] [PubMed:24805361] [PMC:PMC4013143]

About HRH1 / Histamine H1 Receptor

P35367 NM_000861 NP_000852.1

HRH1 Antibody, H1 histamine receptor Antibody, H1 receptor Antibody, Histamine 1 receptor Antibody, Histamine H1 receptor Antibody, H1-R Antibody, H1R Antibody, Histamine H(1) receptor Antibody, Histamine receptor H1 Antibody, HisH1 Antibody

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily.

Immunohistochemistry

Immunohistochemistry
Anti-HRH1 / Histamine H1 Receptor antibody IHC of human small intestine, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1745 concentration 5 ug/ml.

Requested From: 
Date Requested: 12/10/2017

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