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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HRH1 / Histamine H1 Receptor Antibody (aa471‑484) IHC‑plus™ LS‑B1745
Note: This antibody replaces LS-C47564, LS-C67886
Histamine H1 Receptor antibody LS-B1745 is an unconjugated goat polyclonal antibody to Histamine H1 Receptor (HRH1) from human and bat. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Catalog
Size
Price
LS-B1745-50
50 µg (0.5 mg/ml)
Unavailable

Popular HRH1 / Histamine H1 Receptor Products

Antibody
Species: Rat, Mouse, Bovine
Applications: Western blot, ELISA
Antibody
Species: Rat, Mouse, Bovine
Applications: Western blot, ELISA

Product Description

Histamine H1 Receptor antibody LS-B1745 is an unconjugated goat polyclonal antibody to Histamine H1 Receptor (HRH1) from human and bat. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
About HRH1 / Histamine H1 Receptor
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. P35367 NM_000861 NP_000852.1

HRH1 Antibody, H1 histamine receptor Antibody, H1 receptor Antibody, Histamine 1 receptor Antibody, Histamine H1 receptor Antibody, H1-R Antibody, H1R Antibody, Histamine H(1) receptor Antibody, Histamine receptor H1 Antibody, HisH1 Antibody


Specifications

Target
Human HRH1 / Histamine H1 Receptor
Host
Goat
Reactivity
Human, Bat (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide CNENFKKTFKRILH from the C-terminus of human HRH1 / Histamine H1 Receptor (NP_000852.1; NP_001091681.1; NP_001091682.1; NP_001091683.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Bat, Platypus (100%); Monkey, Mouse, Rat, Dog, Bovine, Hamster, Elephant, Panda, Rabbit, Horse, Pig, Turkey, Chicken (93%); Marmoset, Guinea pig, Xenopus (86%).
Blocking Peptide
LS-E25938 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B1745. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa471-484
Specificity
Human HRH1 / Histamine H1 Receptor. Variants (NP_000852.1; NP_001091681.1; NP_001091682.1; NP_001091683.1) encode the same protein.
Usage
Immunohistochemistry: LS-B1745 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1745 was determined to be 2.5 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (2)

1
Estrogenic regulation of histamine receptor subtype H1 expression in the ventromedial nucleus of the hypothalamus in female rats. Mori H, Matsuda K, Yamawaki M, Kawata M. PloS one. 2014 9:e96232. [Full Text Article] [PubMed:24805361] [PMC:PMC4013143]
2
A xenogeneic-free system generating functional human gut organoids from pluripotent stem cells. Uchida H, Machida M, Miura T, Kawasaki T, Okazaki T, Sasaki K, Sakamoto S, Ohuchi N, Kasahara M, Umezawa A, Akutsu H. JCI insight. 2017 2:e86492. (ICC; Human) [Full Text Article] [PubMed:28097227] [PMC:PMC5214546]

Customer Reviews (0)


Images


Immunohistochemistry

Anti-HRH1 / Histamine H1 Receptor antibody IHC of human small intestine, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1745 concentration 5 ug/ml.
Anti-HRH1 / Histamine H1 Receptor antibody IHC of human small intestine, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1745 concentration 5 ug/ml.

Immunohistochemistry

Anti-HRH1 / Histamine H1 Receptor antibody IHC of human small intestine, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1745 concentration 5 ug/ml.
Anti-HRH1 / Histamine H1 Receptor antibody IHC of human small intestine, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1745 concentration 5 ug/ml.

Immunohistochemistry

Anti-HRH1 / Histamine H1 Receptor antibody IHC of human small intestine, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1745 concentration 5 ug/ml.
Anti-HRH1 / Histamine H1 Receptor antibody IHC of human small intestine, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1745 concentration 5 ug/ml.

Immunohistochemistry

Anti-HRH1 / Histamine H1 Receptor antibody IHC of human small intestine, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1745 concentration 5 ug/ml.
Anti-HRH1 / Histamine H1 Receptor antibody IHC of human small intestine, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1745 concentration 5 ug/ml.


Requested From: 
Date Requested: 6/19/2018

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