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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HOXD10 Antibody (aa84‑97) IHC‑plus™ LS‑B9199 Best Seller
Note: This antibody replaces LS-C112742
HOXD10 antibody LS-B9199 is an unconjugated goat polyclonal antibody to HOXD10 from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B9199-50
50 µg (0.5 mg/ml)
Unavailable

Popular HOXD10 Products

Antibody
Species: Mouse, Human
Applications: IHC, Western blot, ELISA
IHC of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HOXD10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Mouse Hoxd10 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded mouse uterus tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Species: Mouse, Human
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry of paraffin-embedded human pancreatic cancer at dilution 1:100
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

HOXD10 antibody LS-B9199 is an unconjugated goat polyclonal antibody to HOXD10 from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About HOXD10
HOXD10 is a member of the Abd-B homeobox family and encodes a protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox D genes located on chromosome 2. The encoded nuclear protein functions as a sequence-specific transcription factor that is expressed in the developing limb buds and is involved in differentiation and limb development. P28358 NM_002148 NP_002139.2

HOXD10 Antibody, Homeo box 4D Antibody, Homeobox protein Hox-D10 Antibody, Hox-4.4 Antibody, Homeobox protein Hox-4E Antibody, HOX4 Antibody, Homeobox D10 Antibody, HOX4D Antibody, HOX4E Antibody, Homeo box D10 Antibody, Homeobox protein Hox-4D Antibody


Specifications

Target
Human HOXD10
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Predicted
Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.2 - 0.6 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HOXD10 antibody was raised against synthetic peptide PNRSCRIEQPVTQQ from an internal region of human HOXD10 (NP_002139.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Tamarin, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Chicken (100%); Opossum, Platypus, Xenopus (93%).
Blocking Peptide
LS-E26853 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B9199. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa84-97
Specificity
Human HOXD10.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx. 37 kDa band observed in Human Kidney lysates (calculated MW of 38.4 kDa according to NP_002139.2). Recommended concentration: 0.2-0.6 ug/ml. Additional bands of unknown identity were also consistently observed at 90+26 kDa. These bands were successfully blocked by incubation with the immunising peptide.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-HOXD10 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-HOXD10 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

HOXD10 antibody (0.2 ug/ml) staining of Human Kidney lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HOXD10 antibody (0.2 ug/ml) staining of Human Kidney lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-HOXD10 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-HOXD10 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

HOXD10 antibody (0.2 ug/ml) staining of Human Kidney lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HOXD10 antibody (0.2 ug/ml) staining of Human Kidney lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-HOXD10 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-HOXD10 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

HOXD10 antibody (0.2 ug/ml) staining of Human Kidney lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HOXD10 antibody (0.2 ug/ml) staining of Human Kidney lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-HOXD10 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-HOXD10 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

HOXD10 antibody (0.2 ug/ml) staining of Human Kidney lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HOXD10 antibody (0.2 ug/ml) staining of Human Kidney lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/21/2018

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