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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HNLF / TMED4 Antibody (aa134‑183) LS‑C80402
TMED4 antibody LS-C80402 is an unconjugated rabbit polyclonal antibody to TMED4 (HNLF) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C80402-50
50 µg
Unavailable

Popular HNLF / TMED4 Products

TMED4 antibody LS-C30631 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Rat, Bovine, Guinea pig, Pig, Xenopus
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Mouse
Applications: Western blot, ELISA
TMED4 antibody LS-C30631 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Rat, Bovine, Guinea pig, Pig, Xenopus
Applications: IHC, IHC - Paraffin, Western blot
TMED4 antibody LS-C30631 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Rat, Bovine, Guinea pig, Pig, Xenopus
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: Immunofluorescence, Western blot, ELISA

Product Description

TMED4 antibody LS-C80402 is an unconjugated rabbit polyclonal antibody to TMED4 (HNLF) from human, mouse, rat and other species. Validated for WB.
About HNLF / TMED4
Involved in vesicular protein trafficking, mainly in the early secretory pathway. targeting. Involved in the maintenance of the Golgi apparatus. Appears to play a role in the biosynthesis of secreted cargo including processing. Involved in endoplasmic reticulum stress response. May play a role in the regulation of heat-shock response and apoptosis. Q7Z7H5 NM_182547 NP_872353.2

TMED4 Antibody, HNLF Antibody, p24 family protein alpha-3 Antibody, ERS25 Antibody, GMP25iso Antibody, p24alpha3 Antibody


Specifications

Target
Human HNLF / TMED4
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Hamster, Horse, Pig, Rabbit, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Drosophila (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
HNLF / TMED4 antibody was raised against synthetic peptide located between aa134-183 of human TMED4 (Q7Z7H5, NP_872353). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Xenopus, Stickleback, Zebrafish (100%); Drosophila, Beetle (92%); Platypus, Lizard (84%).
Blocking Peptide
LS-E15138 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80402. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa134-183
Specificity
Human TMED4
Usage
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 0.5 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Liver cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Liver cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Liver cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Liver cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Liver cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Liver cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Liver cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Liver cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/22/2018

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