(tested or 100% immunogen sequence identity)
HLA-DR15/DR18 antibody was raised against hLA Class 2 Antigen-DR15,18.
Reacts to HLA Class 2 Antigen-DR15,18. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.
Suitable for use in Cell Typing, Tissue Staining, and Chimerism Studies. For a cytotoxic working dilution, add 0.9 ml of 1% BSA in PBS to 100 ul of10X working dilution. Store aliquots at -20?C. Expected Results: Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction. Limitations: Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
Lyophilized powder may be stored at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.