Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Specificity and Use
HLA-DR15/DR16 antibody was raised against hLA Class 2 Antigen-DR15,16.
Reacts to human HLA Class 2 Antigen-DR15,16. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.
Animal Cell Typing, Tissue Staining, and Chimerism Studies. Please refer to the biotinylated versions of selected antibodies for Flow Cytometry applications. FITC-conjugated and purified versions of these antibodies are available on special order. Directions for Use: For a cytotoxic working dilution, add 0.9 ml of 1% BSA in PBS to 100 ul of10X working stock. Store aliquots at -20C. Expected Values: Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction. Limitations: Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results. Storage and Stability (After Reconstitution): May be stored at 4?C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20?C. Aliquots are stable for at least 12 months at -20?C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Lyophilized from PBS, 1% BSA
100 µl 100 ul sterile ddH20
Store at -20 to -70 C. Stable for 12 months from date of receipt.