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Anti-HLA-DR Antibody (clone YE2/36 HLK, FITC) LS-C58632

Catalog Size Price
LS-C58632-100 100 µg Unavailable

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100% Guaranteed
Rat Monoclonal [clone YE2/36 HLK] (IgG2a) to Human HLA-DR
Human
Flow Cytometry
FITC Conjugated

Details

Human HLA-DR
Rat
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [YE2/36 HLK]
Fluorescein
Affinity purified
Unmodified

Applications

Flow Cytometry (1:1 - 1:10)

Specificity and Use

HLA-DR antibody was raised against eHR-B Ramos cells.
Recognizes a monomorphic determinant of human HLA-DR antigen (MHC class II), expressed by B lymphocytes, activated T cells and by monocytes. Clone YE2/36 has also been reported to be cross reactive with mouse H-2 haplotypes b, d and q expressed by mouse strains including C57BL/6, DBA, and NZB. This clone has been described reacting with paraffin embedded material following PLP (periodate-lysine-paraformaldehyde) fixation. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

0.09% sodium azide, 1% BSA.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About HLA-DR

HLA-DR is an MHC class II cell surface receptor encoded by the human leukocyte antigen complex on chromosome 6 region 6p21.31. The complex of HLA-DR and its ligand, a peptide of 9 amino acids in length or longer, constitutes a ligand for the T-cell receptor (TCR). HLA (human leukocyte antigens) were originally defined as cell surface antigens that mediate graft-versus-host disease, which resulted in the rejection of tissue transplants in HLA-mismatched donors.

Requested From: 
Date Requested: 5/23/2018

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