Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal to Drosophila melanogaster Histone H2AvD
Western blot, ELISA
Drosophila melanogaster Histone H2AvD
Drosophila melanogaster (tested or 100% immunogen sequence identity)
Western blot (1:400 - 1:1600)
ELISA (1:4000 - 1:16000)
Specificity and Use
Histone H2AvD antibody was raised against synthetic peptide corresponding to amino acids 132-141 of Drosophila melanogaster (fruit fly) H2AvD protein.
the phosphorylated form of Drosophila H2AvD protein at the pS137 residue. Reactivity occurs against Drosophila H2AvD pS137 protein and Reactivity with non-phosphorylated Drosophila H2AvD is minimal by ELISA. A BLAST analysis was used to suggest little to no cross reactivity with H2AvD proteins from other sources based on a comparison using the immunizing sequence. Reactivity against homologs from other sources is not known
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 14 kD in size corresponding to phosphorylated H2AvD protein by western blotting in the appropriate Drosophila tissue or cell lysate or extract. Less than 0.2% reactivity is observed against the non-phosphorylated form of the immunizing peptide.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Aliquot and store at -20°C. Minimize freezing and thawing.
Anti-Histone H2AvD pS137 Antibody - Western Blot. Western blot of affinity purified anti-histone H2AvD pS137 antibody shows detection of a band at ~15 kD corresponding to phosphorylated H2AvD (lane 2 arrow-head). Lanes contain either mock-irradiated (lane 1) or 4000-RAD gamma irradiated (lane 2) Drosophila melanogaster (3rd instar) larvae brain WC lysate separated on by SDS-PAGE and transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Washes and reaction with secondary antibody followed incubation. Use HRP conjugated Gt-a-Rabbit IgG [H&L] MX (LS-C60865) and ECL for detection. Personal Communication. Yikang Rong, NIH, CCR, Bethesda, MD.