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Anti-Histone H1 Antibody (clone AE-4) IHC-plus™ LS-B4038

Note: This antibody replaces LS-C27228

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Related Products

Most Popular Histone H1 Antibodies

Anti-Histone H1 Antibody LS-C24255
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Anti-Histone H1 Antibody (clone AE-4) IHC-plus™ LS-B4038
Mouse Monoclonal [clone AE-4] (IgG2a) to Human Histone H1
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IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-Histone H1 Antibody (N-Terminus) IHC-plus™ LS-B13719
Rabbit Polyclonal to Human Histone H1
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IHC - Paraffin, ICC, Immunofluorescence, Western blot
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Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone AE-4] (IgG2a) to Human Histone H1
Human
IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human Histone H1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [AE-4]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • IHC - Frozen
  • Immunofluorescence
  • Western blot (1:100)
  • ELISA

Specificity and Use

Histone H1 antibody was raised against nuclei from myeloid leukemia biopsy cells.
Recognizes histone H1. The histone proteins serve as a structural scaffold for the organization of nuclear DNA into chromatin. In eukaryotic cells there are 4 core histone proteins, H2A, H2B, H3 and H4 that assemble to form an octamer round which the DNA is wrapped in a sequence dependant manner. The resulting complex is known as a nucleosome and is the basic subunit of chromatin. Histone H1 acts as a linker and binds to each nucleosome, and by self affinity, links these nucleosomes together resulting in the 30 nm chromatin fiber. In blots of rat brain homogenate a duplet is observed at 27-29 kD consistent with Histone H1. Speckled staining is observed in the nucleus of normal and malignant cells.
Immunohistochemistry: LS-B4038 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4038 was determined to be 5 ug/ml.

Packaging

PBS, 0.09% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About Histone H1

Histone H1 is one of the five main histone protein families which are components of chromatin in eukaryotic cells. Though highly conserved, it is nevertheless the most variable histone in sequence across species. Metazoan H1 proteins feature a central globular domain and long C- and short N-terminal tails. H1 is involved with the packing of the "beads on a string" sub-structures into a high order structure, whose details have not yet been solved.

Immunohistochemistry

Immunohistochemistry
Anti-Histone H1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4038 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-Histone H1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4038 concentration 5 ug/ml.

Requested From: United States
Date Requested: 12/5/2016

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