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Anti-HIST1H3E Antibody (phospho-Ser10) LS-C96882


Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

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Rabbit Polyclonal to Human HIST1H3E
IHC - Paraffin, Dot Blot


Human HIST1H3E
Human (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (1:50 - 1:100)
  • Dot Blot (1:500)

Specificity and Use

HIST1H3E antibody was raised against kLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human HIST1H3B3.
LS-E7276 - Lyophilized - 100 µg - $145.00


PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About HIST1H3E

NM_003532 NP_003523.1

HIST1H3E Antibody, H3.1 Antibody, Histone 1, H3e Antibody, Histone cluster 1, H3e Antibody, Histone H3.1 Antibody

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures.


Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

Western blot
Western blot of lysate from HeLa cell line, using Phospho-H3-pS10 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Western blot

Western blot
Western blot of extracts from HeLa cells,untreated or treated with FBS and Calyculin A,100nM, using phospho-H3-Ps10(left) or H3 antibody(right)

Western blot

Western blot
Western blot of extracts from HeLa cells,untreated or treated with FBS and Calyculin A ,100nM, using phospho-HIST1H3B3-S10(left) or HIST1H3B3 antibody(right)

Dot Blot

Dot Blot
Dot blot of anti-Phospho-HIST1H3B3-S10 Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Requested From: United States
Date Requested: 10/26/2016

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