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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HIST1H2AB Antibody (aa12‑40) LS‑C203664
HIST1H2AB antibody LS-C203664 is an unconjugated rabbit polyclonal antibody to human HIST1H2AB. Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C203664-400
400 µl
Unavailable

Popular HIST1H2AB Products

HIST1H2AB Antibody western blot of K562 cell line lysates (35 ug/lane). The HIST1H2AB antibody detected the HIST1H2AB protein (arrow).
Species: Human
Applications: Western blot
Antibody
Species: Human
Applications: ICC, Western blot, ELISA
Immunofluorescence staining of Hela cells diluted at 1:168,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
Immunocytochemistry analysis diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: ICC, Immunofluorescence, ELISA
Western Blot Positive WB detected in:Mouse spleen tissue All Lanes:Hydroxyl-Histone H2A type 1-B/E (Y39) antibody at 1.05µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 14 KDa Observed band size: 14 KDa
Species: Human
Applications: Western blot, ELISA

Product Description

HIST1H2AB antibody LS-C203664 is an unconjugated rabbit polyclonal antibody to human HIST1H2AB. Validated for Flow, IHC and WB.
About HIST1H2AB
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. NM_003513

HIST1H2AB Antibody


Specifications

Target
Human HIST1H2AB
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Chicken, Xenopus, Zebrafish, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Epitope
aa12-40
Specificity
This HIST1H2AB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-40 amino acids from the N-terminal region of human HIST1H2AB.
Presentation
PBS, 0.09% sodium azide.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

HIST1H2AB Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human cerebellum tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
HIST1H2AB Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human cerebellum tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

HIST1H2AB Antibody western blot of K562,CEM,MDA-MB453,NCI-H460,MDA-MB231 cell line lysates (35 ug/lane). The HIST1H2AB antibody detected the HIST1H2AB protein (arrow).
HIST1H2AB Antibody western blot of K562,CEM,MDA-MB453,NCI-H460,MDA-MB231 cell line lysates (35 ug/lane). The HIST1H2AB antibody detected the HIST1H2AB protein (arrow).

Flow Cytometry

HIST1H2AB Antibody flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
HIST1H2AB Antibody flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

HIST1H2AB Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human cerebellum tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
HIST1H2AB Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human cerebellum tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

HIST1H2AB Antibody western blot of K562,CEM,MDA-MB453,NCI-H460,MDA-MB231 cell line lysates (35 ug/lane). The HIST1H2AB antibody detected the HIST1H2AB protein (arrow).
HIST1H2AB Antibody western blot of K562,CEM,MDA-MB453,NCI-H460,MDA-MB231 cell line lysates (35 ug/lane). The HIST1H2AB antibody detected the HIST1H2AB protein (arrow).

Flow Cytometry

HIST1H2AB Antibody flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
HIST1H2AB Antibody flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

HIST1H2AB Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human cerebellum tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
HIST1H2AB Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human cerebellum tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

HIST1H2AB Antibody western blot of K562,CEM,MDA-MB453,NCI-H460,MDA-MB231 cell line lysates (35 ug/lane). The HIST1H2AB antibody detected the HIST1H2AB protein (arrow).
HIST1H2AB Antibody western blot of K562,CEM,MDA-MB453,NCI-H460,MDA-MB231 cell line lysates (35 ug/lane). The HIST1H2AB antibody detected the HIST1H2AB protein (arrow).

Flow Cytometry

HIST1H2AB Antibody flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
HIST1H2AB Antibody flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

HIST1H2AB Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human cerebellum tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
HIST1H2AB Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human cerebellum tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

HIST1H2AB Antibody western blot of K562,CEM,MDA-MB453,NCI-H460,MDA-MB231 cell line lysates (35 ug/lane). The HIST1H2AB antibody detected the HIST1H2AB protein (arrow).
HIST1H2AB Antibody western blot of K562,CEM,MDA-MB453,NCI-H460,MDA-MB231 cell line lysates (35 ug/lane). The HIST1H2AB antibody detected the HIST1H2AB protein (arrow).

Flow Cytometry

HIST1H2AB Antibody flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
HIST1H2AB Antibody flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/25/2018

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