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Antibodies > HIF2A / EPAS1 > LS-C343868
 
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Catalog Number Size Price
LS-C343868-10 10 µg $318 
LS-C343868-100 100 µg (0.5 mg/ml) $470 
HIF2A / EPAS1 Antibody - Western blot analysis of HIF-2A using anti-HIF-2A antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat RH35 whole cell lysates, Lane 3: rat small intestine tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF-2A antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HIF-2A at approximately 120KD. The expected band size for HIF-2A is at 96KD.
HIF2A / EPAS1 Antibody - Western blot analysis of HIF2A using anti-HIF2A antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat testis tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF2A antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HIF2A at approximately 110-120KD. The expected band size for HIF2A is at 96KD.
HIF2A / EPAS1 Antibody - Western blot analysis of HIF-2A using anti-HIF-2A antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse lun tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF-2A antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HIF-2A at approximately 120KD. The expected band size for HIF-2A is at 96KD.
HIF2A / EPAS1 Antibody - HIF-2-alpha antibody Western blot. Lane 1: HELA Cell Lysate. Lane 2: MCF-7 Cell Lysate. Lane 3: JURKAT Cell Lysate.
HIF2A / EPAS1 Antibody - Western blot analysis of HIF-2A using anti-HIF-2A antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF-2A antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HIF-2A at approximately 120KD. The expected band size for HIF-2A is at 96KD.
HIF2A / EPAS1 Antibody - Western blot analysis of HIF-2A using anti-HIF-2A antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat RH35 whole cell lysates, Lane 3: rat small intestine tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF-2A antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HIF-2A at approximately 120KD. The expected band size for HIF-2A is at 96KD.
HIF2A / EPAS1 Antibody - Western blot analysis of HIF2A using anti-HIF2A antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat testis tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF2A antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HIF2A at approximately 110-120KD. The expected band size for HIF2A is at 96KD.
HIF2A / EPAS1 Antibody - Western blot analysis of HIF-2A using anti-HIF-2A antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse lun tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF-2A antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HIF-2A at approximately 120KD. The expected band size for HIF-2A is at 96KD.
HIF2A / EPAS1 Antibody - HIF-2-alpha antibody Western blot. Lane 1: HELA Cell Lysate. Lane 2: MCF-7 Cell Lysate. Lane 3: JURKAT Cell Lysate.
HIF2A / EPAS1 Antibody - Western blot analysis of HIF-2A using anti-HIF-2A antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF-2A antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HIF-2A at approximately 120KD. The expected band size for HIF-2A is at 96KD.
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Polyclonal Rabbit anti‑Human HIF2A / EPAS1 Antibody (aa282‑301, WB) LS‑C343868

Polyclonal Rabbit anti‑Human HIF2A / EPAS1 Antibody (aa282‑301, WB) LS‑C343868

Submit Review LSBio Guarantee
Note: This antibody replaces LS-C388570
Antibody:
HIF2A / EPAS1 Rabbit anti-Human Polyclonal (aa282-301) Antibody
Application:
WB
Reactivity:
Human, Orangutan, Monkey, Mouse, Rat, Bovine, Horse, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C343868-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
HIF2A / EPAS1 Rabbit anti-Human Polyclonal (aa282-301) Antibody
Application:
WB
Reactivity:
Human, Orangutan, Monkey, Mouse, Rat, Bovine, Horse, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
EPAS1 antibody LS-C343868 is an unconjugated rabbit polyclonal antibody to EPAS1 (HIF2A) (aa282-301) from human. It is reactive with human, mouse, rat and other species. Validated for WB.
Target
Human HIF2A / EPAS1
Synonyms
EPAS1 | BHLHe73 | ECYT4 | HIF-1-alpha-like factor | HIF2-alpha | HIF2A | EPAS-1 | HIF-2-alpha | HIF-1alpha-like factor | MOP2 | Member of PAS protein 2 | PASD2
Host
Rabbit
Reactivity
Human, Orangutan, Monkey, Mouse, Rat, Bovine, Horse, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human HIF-2-alpha(282-301 aa , HALDSENMTKSHQNLCTKGQ), identical to the related mouse and rat sequence.
Epitope
aa282-301
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIF2A / EPAS1
Q99814 NM_001430 NP_001421.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024
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