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Anti-HFE Antibody (clone 3F1) LS-C169421

Catalog Size Price
LS-C169421-100 100 µl Unavailable

Related Products

35 HFE Antibodies

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Anti-HFE Antibody (aa115-206) LS-C129759
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Anti-HFE Antibody (clone 3F1) LS-C169421
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Anti-HFE Antibody (clone 1G12) LS-C197405
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100% Guaranteed
Mouse Monoclonal [clone 3F1] (IgG1) to Human HFE
Human
IHC, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human HFE
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [3F1]
Unconjugated
Ascites
Unmodified

Applications

  • IHC
  • ICC (1:200 - 1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000)

Specificity and Use

HFE antibody was raised against purified recombinant fragment of human HFE expressed in E. coli.
Human HFE

Packaging

Ascites, 0.03% sodium azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About HFE

Q30201 NM_000410 NP_000401.1

HFE Antibody, HFE1 Antibody, HH Antibody, MHC class I-like protein HFE Antibody, Hemochromatosis Antibody, High Fe Antibody, MVCD7 Antibody, HLA-H Antibody, HLAH Antibody, TFQTL2 Antibody

HFE is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene.

Immunofluorescence

Immunofluorescence
Immunofluorescence of HepG2 cells using HFE mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Western blot

Western blot
Western blot using HFE monoclonal antibody against HEK293 (1) and HFE(AA: 125-282)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot

Western blot
Western blot using HFE monoclonal antibody against human HFE (AA: 125-282) recombinant protein. (Expected MW is 44 kDa)

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Requested From: 
Date Requested: 12/11/2017

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