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Anti-Hemagglutinin / HA Tag Antibody (C-Terminus) LS-C50852

Ordering

Wt. Vol. Conc. Price
- 500 µl - Unavailable

Most Popular Hemagglutinin / HA Tag Antibodies

Anti-Hemagglutinin / HA Tag Antibody (C-Terminus) LS-C50851
Chicken Polyclonal (IgY)
IHC, Western blot, ELISA
Unconjugated
Anti-Hemagglutinin / HA Tag Antibody (C-Terminus) LS-C50863
Mouse Monoclonal (IgG1)
IHC, ICC, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Anti-Hemagglutinin / HA Tag Antibody LS-C136213
Sheep Polyclonal (IgG)
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG)
Western blot, Immunoprecipitation (applications tested for the base form of this product only)
Unconjugated

Details

Hemagglutinin / HA Tag
Rabbit
IgG Polyclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, MaxLight 405, MaxLight 490, MaxLight 550, MaxLight 650, MaxLight 750, PE.
Protein A purified
Unmodified

Applications

  • Western blot (5 - 10 µg/ml)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)

Specificity and Use

Synthetic peptide containing the sequence YPYDVPDYAASL derived from the influenza virus hemaglutinin (HA) protein (KLH coupled). Cellular Localization: Depends upon the localization of the parent protein tagged with the HA tag.
C-Terminus
Recognizes recombinant proteins containing the HA epitope tag: YPYDVPDYA. Recognizes the HA-tag fused to either the N- or C-termini of targeted proteins. Species cross-reactivity: A broad species cross-reactivity is expected based on conservation of sequence homology.
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 5-10 ug/ml for 2hrs at RT. Immunoprecipitation: 10 ug/mg protein lysate. Use Protein A. Positive control: Proteins displaying the YPYDVPDYA HA-tag.

Packaging

PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About Hemagglutinin / HA Tag

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.

Requested From: 
Date Requested: 3/28/2017

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