HELT antibody LS-C82614 is an unconjugated rabbit polyclonal antibody to HELT from human, mouse, rat and other species. Validated for WB.
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Rabbit, Zebrafish
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
- Western blot
- (applications tested for the base form of this product only)
HELT antibody was raised against synthetic peptide located between aa141-190 of human HELT (A6NFD8, NP_001025058). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Panda, Bovine, Bat, Rabbit, Horse, Opossum, Guinea pig, Zebra finch, Lizard, Stickleback, Zebrafish (100%); Elephant, Dog (92%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C82614. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.