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Anti-HEC1 / NDC80 Antibody (aa56-642, clone 9G3.23) IHC-plus™ LS-B1704

Note: This antibody replaces LS-C20070
Catalog Size Price
LS-B1704-50 50 µl (0.27 mg/ml) Unavailable

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100% Guaranteed
Mouse Monoclonal [clone 9G3.23] (IgG) to Human HEC1 / NDC80
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation


Human HEC1 / NDC80
Human (tested or 100% immunogen sequence identity)
IgG Monoclonal [9G3.23]
Protein G purified


  • IHC - Paraffin (1:50)
  • Immunofluorescence
  • Western blot (1:500 - 1:3000)
  • Immunoprecipitation

Specificity and Use

HEC1 / NDC80 antibody was raised against recombinant human NDC80 / HEC1.
Human HEC1 protein consisting of amino acids 56-642.
Immunohistochemistry: LS-B1704 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B1704 was determined to be 1:50.


+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About HEC1 / NDC80

O14777 NM_006101 NP_006092.1

NDC80 Antibody, HEC1 Antibody, Kinetochore protein Hec1 Antibody, Kinetochore associated 2 Antibody, HEC Antibody, HsHec1 Antibody, HsNDC80 Antibody, KNTC2 Antibody, TID3 Antibody

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.


Anti-NDC80 / HEC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1704 concentration 10 ug/ml.

Requested From: 
Date Requested: 3/24/2018

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