Vigilin antibody LS-B1575 is an unconjugated rabbit polyclonal antibody to Vigilin (HDLBP) from human, rat, bat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Human HDLBP / Vigilin
Human, Monkey, Rat, Bat, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
- IHC - Paraffin (5 µg/ml)
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
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HDLBP / Vigilin antibody was raised against synthetic peptide from N-Terminus of human HDLBP (Q00341, NP_005327). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Rat, Hamster, Elephant, Dog, Bat, Rabbit, Horse, Pig, Guinea pig (100%); Mouse, Bovine, Opossum, Turkey, Chicken (92%); Platypus, Lizard (84%); Xenopus (81%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B1575. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunohistochemistry: LS-B1575 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1575 was determined to be 5 ug/ml.
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.