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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HDAC5 Antibody (aa536‑545) LS‑C7360
HDAC5 antibody LS-C7360 is an unconjugated rabbit polyclonal antibody to HDAC5 from human, bat, bovine and other species. Validated for WB.
Antibody
Catalog
Size
Price
LS-C7360-200
200 µg
$710

Popular HDAC5 Products

Anti-HDAC5 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Western blot of HDAC5 in 293 (lane 1), Jurkat (lane 2) and NIH-3T3 (lane 3) cell lysate with antibody.
Species: Human, Mouse
Applications: Western blot, Immunoprecipitation, Chromatin Immunoprecipitation
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HDAC5 Antibody. The picture on the right is blocked with the synthesized peptide.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemistry: HDAC5 Antibody - IHC analysis of HDAC5 in mouse pancreas.
Species: Human, Mouse, Primate
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Immunohistochemical analysis of Histone Deacetylase 5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Bovine, Dog, Pig
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot

Product Description

HDAC5 antibody LS-C7360 is an unconjugated rabbit polyclonal antibody to HDAC5 from human, bat, bovine and other species. Validated for WB.

Specifications

Target
Human HDAC5
Synonyms
HDAC5, Antigen NY-CO-9, Histone deacetylase 5, NY-CO-9, HD5, KIAA0600
Host
Rabbit
Reactivity
Human, Monkey, Bat, Bovine, Dog, Hamster, Horse, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
HDAC5 antibody was raised against kLH-conjugated synthetic peptides corresponding to amino acids 536-545 and 194-206 (C-TKTGELPRQP and C-KSKEPTPGGLNHS) of human HDAC5. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Hamster, Elephant, Porcine, Dog, Bat, Bovine, Horse, Rabbit, Opossum (100%); Mouse, Rat, Lizard, Panda (90%).
Epitope
aa536-545
Specificity
Recognizes HDAC5, Mr 160kD. Species cross-reactivity: Human. Weak cross-reactivity with rat and mouse.
Usage
Suitable for use in Western Blot. Western Blot:1-4 ug/ul detects HDAC5 from a HeLa nuclear extract. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HDAC5 (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About HDAC5
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. Q9UQL6 NM_005474 NP_005465.2


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Requested From: United States
Date Requested: 11/15/2018

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