HDAC5 antibody LS-C7360 is an unconjugated rabbit polyclonal antibody to HDAC5 from human, bat, bovine and other species. Validated for WB.
Human, Monkey, Bat, Bovine, Dog, Hamster, Horse, Rabbit
(tested or 100% immunogen sequence identity)
Mouse, Rat (at least 90% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot
- (applications tested for the base form of this product only)
HDAC5 antibody was raised against kLH-conjugated synthetic peptides corresponding to amino acids 536-545 and 194-206 (C-TKTGELPRQP and C-KSKEPTPGGLNHS) of human HDAC5. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Hamster, Elephant, Porcine, Dog, Bat, Bovine, Horse, Rabbit, Opossum (100%); Mouse, Rat, Lizard, Panda (90%).
Recognizes HDAC5, Mr 160kD. Species cross-reactivity: Human. Weak cross-reactivity with rat and mouse.
Suitable for use in Western Blot. Western Blot:1-4 ug/ul detects HDAC5 from a HeLa nuclear extract. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HDAC5 (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.