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Anti-HDAC3 Antibody (aa2-17) IHC-plus™ LS-B2032

Note: This antibody replaces LS-C690, LS-C67221

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular HDAC3 Antibodies

Anti-HDAC3 Antibody (aa224-428, clone 3A7B5) IHC-plus™ LS-B3893
Mouse Monoclonal [clone 3A7B5] (IgG1) to Human HDAC3
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human HDAC3
Human
IHC - Paraffin, Western blot, Immunoprecipitation, Chromatin Immunoprecipitation
Unconjugated

Details

Human HDAC3
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (2 µg/ml)
  • Immunoprecipitation
  • Chromatin Immunoprecipitation

Specificity and Use

HDAC3 antibody was raised against synthetic peptide from human HDAC3.
aa2-17
In HeLa, a 50 kD band is observed.
Immunohistochemistry: LS-B2032 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2032 was determined to be 10 ug/ml.

Packaging

PBS, 0.2% gelatin, 0.05% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About HDAC3

O15379 NM_003883 NP_003874.2

HDAC3 Antibody, RPD3 Antibody, SMAP45 Antibody, HD3 Antibody, Histone deacetylase 3 Antibody, RPD3-2 Antibody

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.

Immunohistochemistry

Immunohistochemistry
Anti-HDAC3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2032 concentration 10 ug/ml.

Western blot

Western blot
Western blot of HDAC3 in HeLa cell lysate with anti-HDAC3 antibody.

Requested From: United States
Date Requested: 12/10/2016

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