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Anti-HDAC1 Antibody (aa466-482) IHC-plus™ LS-B34

Note: This antibody replaces LS-C18818, LS-C56878

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HDAC1 Antibodies

Anti-HDAC1 Antibody (aa466-482) IHC-plus™ LS-B34
Rabbit Polyclonal to Human HDAC1
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-HDAC1 Antibody (aa467-482) IHC-plus™ LS-B3438
Mouse Monoclonal (IgG1) to Human HDAC1
Human, Monkey, Mouse, Hamster, Rabbit
IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-HDAC1 Antibody (clone 3E1) IHC-plus™ LS-B6094
Mouse Monoclonal [clone 3E1] (IgG1,k) to Human HDAC1
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human HDAC1
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human HDAC1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (20 µg/ml)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:10000 - 1:50000)

Specificity and Use

HDAC1 antibody was raised against synthetic peptide from human HDAC1.
aa466-482
Amino acids 466-482 of Human HDAC-1
Immunohistochemistry: LS-B34 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B34 was determined to be 20 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About HDAC1

Q13547 NM_004964 NP_004955.2

HDAC1 Antibody, Histone deacetylase 1 Antibody, GON-10 Antibody, HD1 Antibody, RPD3 Antibody, RPD3L1 Antibody

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function.

Immunohistochemistry

Immunohistochemistry
Anti-HDAC1 antibody IHC of human prostate carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B34 concentration 20 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence Microscopy - HDAC Antibody. Histone deacetylase (HDAC) antibody detects HDAC (colored GREEN) as used in STED immunofluorescence microscopy. Methanol fixed A431 cells were blocked with normal goat serum. The cells were then probed with 0.4 ug/mL final concentration of anti-HDAC and detected with 0.2 ug/mL DyLight488 conjugated Anti-RABBIT IgG [GOAT] secondary antibody. Also shown in this 2-color STED image is an anti-tubulin monoclonal antibody [MOUSE] ( detected with ATTO 425 conjugated anti-MOUSE IgG [GOAT] ( secondary antibody (colored RED).

Immunofluorescence

Immunofluorescence
Anti-HDAC-1 polyclonal antibody-Immunofluorescence Microscopy. polyclonal Anti-HDAC-1 antibody (LS-B34) was used with Atto 425 Anti-Rabbit IgG (shown in red) for to detect HDAC-1 by immunofluorescence. Anti-Keratin monoclonal antibody was used with Dylight 488 goat anti-mouse shown in green) to detect Keratin by Immunofluorescence. Data was collected on a STED-CW TCS-SP5 Confocal system (Leica Microsystems) equipped with a DFC 350FX Camera allowing sequential acquisition in wide-field, confocal and STED CW imaging modes and provided courtesy of: Myriam Gastard, PhD, personal communication, Leica Microsystems, Inc. USA.

Western blot

Western blot
Anti-HDAC-1 Antibody - Western Blot. p/n LS-B34 was used for Western blot of LNCaP prostate cancer cells. Lysate was loaded at 50 ug per lane and incubated with 1:1000 dilution of primary Ab (Lot. 16248). A band was detected at the expected molecular weight of 55 Kda. Personal communication Flavio Rizzolio, Temple University.

Western blot

Western blot
Anti-HDAC-1 Antibody - Western Blot. Western blot of Affinity Purified anti-HDAC-1 antibody shows detection of a band at ~65 kD corresponding to human HDAC1 present in a 293 whole cell lysate (arrowhead). Approximately 35 ug of lysate was separated on a 4-20% Tris-HEPES gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1350. Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
 

Requested From: United States
Date Requested: 12/5/2016

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