Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone A1/3D1] (IgG1,k) to HCV / Hepatitis C Virus
Hepatitis C Virus
Western blot, ELISA
HCV / Hepatitis C Virus
Hepatitis C Virus (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [A1/3D1]
Protein G purified
Western blot (0.5 - 1 µg/ml)
Specificity and Use
Synthetic peptides derived from HCV capsid protein.
Reactive with Recombinant Capsid Protein C and Envelop Protein M (core) (1-142aa) and Synthetic Capsid Protein C (1-61aa). Recognizes different antigenic determinants of HCV capsid protein. Does not cross-react with recombinant or synthetic HCV non-structural proteins (NS-3 and NS-4).
Suitable for use in ELISA and Western Blot. ELISA: Indirect ELISA method. The testing plates were coated with HCV Non-Structural Protein (NS), Capsid Protein (C) and the mixture of NS and C separately. The neat culture supernatant of hybridoma showed a strong reaction with HCV C (OD492 value >2) and mixture of NS and C (OD492 value >1.5). Showed no reaction with HCV NS (OD492 value < 0.03). Western Blot: 0.5-1 ug/ml will allow visualization 0.1 ug/lane (Recombinant CPC+EPM (core)), 0.5 ug/lane (Synthetic CPC), and 0.1 ug/lane (Recombinant Chimeric HCV Polyprotein). Testing is under reducing and non-reducing conditions.
PBS, pH 7.2, 40% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.