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Anti-HCRT / Orexin Antibody IHC-plus™ LS-B31

Note: This antibody replaces LS-C11429

Ordering

Wt. Vol. Conc. Price
- 50 µl - Unavailable

Most Popular HCRT / Orexin Antibodies

Anti-HCRT / Orexin Antibody IHC-plus™ LS-B31
Sheep Polyclonal to Human HCRT / Orexin
Human
IHC - Paraffin, IHC - Frozen, ELISA
Unconjugated
Immunohistochemistry Image
Anti-HCRT / Orexin Antibody (aa2-14) LS-C122252
Sheep Polyclonal (IgG) to Human HCRT / Orexin
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, IHC - Frozen, ELISA
Unconjugated
Anti-HCRT / Orexin Antibody (aa12-33) LS-C124312
Rabbit Polyclonal to Bovine HCRT / Orexin
Bovine, Human, Monkey, Mouse, Rat, Bat, Dog, Hamster, Horse, Pig, Rabbit
IHC
Unconjugated

100% Guaranteed 100% Guaranteed
Sheep Polyclonal to Human HCRT / Orexin
Human
IHC - Paraffin, IHC - Frozen, ELISA
Unconjugated

Details

Human HCRT / Orexin
Sheep
Human (tested or 100% immunogen sequence identity)
Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit, Rhesus monkey (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen
  • ELISA

Specificity and Use

HCRT / Orexin antibody was raised against synthetic peptide, PLPDCCRQKTCSC, corresponding to amino acids 2-14 of human orexin A conjugated to KLH.
Human orexin A, an excitatory neuropeptide linked to alertness and wakefulness in humans.
Immunohistochemistry: LS-B31 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B31 was determined to be 10 mg/ml. Histology Positive Control Tissue: Hypothalamus.

Packaging

PBS, pH 7.2, 0.09% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About HCRT / Orexin

O43612 NM_001524 NP_001515.1

HCRT Antibody, PPORX Antibody, OX Antibody, Hypocretin Antibody, NRCLP1 Antibody, Orexin Antibody

Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested.

Immunohistochemistry

Immunohistochemistry
Anti-HCRT / Orexin antibody IHC of human brain, hypothalamus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B31 concentration 10 ug/ml.
 

Requested From: 
Date Requested: 3/25/2017

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