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Anti-HCA557B / METTL21A Antibody (N-Terminus) LS-C102481

Catalog	Size	Price
LS-C102481-50	50 µg	Unavailable

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Reactivity:	Human, Monkey, Mouse, Bat, Dog, Guinea pig, Hamster, Rabbit
Application:	Western blot
Format:	Unconjugated

Anti-HCA557B / METTL21A Antibody (clone 2D6) LS-C173386

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Reactivity:	Human
Application:	Western blot
Format:	Unconjugated

Anti-HCA557B / METTL21A Antibody (clone 2G7) LS-C173387

Antibody:	Mouse Monoclonal [clone 2G7] (IgG2b) to Human HCA557B / METTL21A
Reactivity:	Human
Application:	IHC - Paraffin, Western blot, Flow Cytometry
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Anti-HCA557B / METTL21A Antibody (clone 2D3) LS-C173448

Antibody:	Mouse Monoclonal [clone 2D3] (IgG2b) to Human HCA557B / METTL21A
Reactivity:	Human
Application:	Immunofluorescence, Western blot
Format:	Unconjugated

Anti-HCA557B / METTL21A Antibody (clone 1C8) LS-C337550

Antibody:	Mouse Monoclonal [clone 1C8] (IgG2a) to Human HCA557B / METTL21A
Reactivity:	Human
Application:	Western blot
Format:	Unconjugated

Antibody:	Rabbit Polyclonal (IgG) to Human HCA557B / METTL21A
Reactivity:	Human, Monkey, Mouse, Bat, Dog, Guinea pig, Hamster, Rabbit
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

Details

Target:	Human HCA557B / METTL21A
Host:	Rabbit
Reactivity:	Human, Monkey, Mouse, Bat, Dog, Guinea pig, Hamster, Rabbit (tested or 100% immunogen sequence identity)
Predicted:	Rat, Bovine, Pig (at least 90% immunogen sequence identity)
Clonality:	IgG Polyclonal
Conjugations:	Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot (0.2 - 1 µg/ml)
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

HCA557B / METTL21A antibody was raised against synthetic peptide from N-Terminus of human METTL21A (Q8WXB1, NP_660323). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Elephant, Panda, Dog, Bat, Rabbit, Guinea pig (100%); Orangutan, Galago, Rat, Bovine, Pig (92%); Horse (85%).

Blocking Peptide:

LS-E19042 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C102481. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

N-Terminus

Specificity:

Human METTL21A / FAM119A

Usage:

Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Packaging

Presentation:	Lyophilized from PBS with 2% sucrose
Reconstitution:	Distilled water
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About HCA557B / METTL21A

NCBI: AA701163

Synonyms: METTL21A Antibody, HCA557b Antibody, FAM119A Antibody, LOC151194 Antibody, Methyltransferase like 21A Antibody

Description: Protein-lysine methyltransferase that selectively trimethylates residues in heat shock protein 70 (HSP70) family members. Contributes to the in vivo trimethylation of Lys residues in HSPA1 and HSPA8. In vitro methylates 'Lys-561' in HSPA1, 'Lys-564' in HSPA2, 'Lys-585' in HSPA5, 'Lys-563' in HSPA6 and 'Lys-561' in HSPA8.

(More About HCA557B / METTL21A)

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Western blot

METTL21A / FAM119A antibody LS-C102481 Western blot of Fetal Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 6/28/2017

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