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HCA557B / METTL21A Antibody (N‑Terminus) LS‑C102481
METTL21A antibody LS-C102481 is an unconjugated rabbit polyclonal antibody to METTL21A (HCA557B) from human, mouse, bat and other species. Validated for WB.
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Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Species:
Human
Applications:
Immunofluorescence, Western blot
Product Description
METTL21A antibody LS-C102481 is an unconjugated rabbit polyclonal antibody to METTL21A (HCA557B) from human, mouse, bat and other species. Validated for WB.
About HCA557B / METTL21A
Protein-lysine methyltransferase that selectively trimethylates residues in heat shock protein 70 (HSP70) family members. Contributes to the in vivo trimethylation of Lys residues in HSPA1 and HSPA8. In vitro methylates 'Lys-561' in HSPA1, 'Lys-564' in HSPA2, 'Lys-585' in HSPA5, 'Lys-563' in HSPA6 and 'Lys-561' in HSPA8.
AA701163
METTL21A Antibody, HCA557b Antibody, FAM119A Antibody, LOC151194 Antibody, Methyltransferase like 21A Antibody
Specifications
Target
Human HCA557B / METTL21A
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Bat, Dog, Guinea pig, Hamster, Rabbit
(tested or 100% immunogen sequence identity)
Predicted
Rat, Bovine, Pig (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
Immunogen
Synthetic peptide from N-Terminus of human METTL21A (Q8WXB1, NP_660323). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Elephant, Panda, Dog, Bat, Rabbit, Guinea pig (100%); Orangutan, Galago, Rat, Bovine, Pig (92%); Horse (85%).
Blocking Peptide
LS-E19042 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C102481. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-C102481. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human METTL21A / FAM119A
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Western blot
METTL21A / FAM119A antibody LS-C102481 Western blot of Fetal Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
METTL21A / FAM119A antibody LS-C102481 Western blot of Fetal Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
METTL21A / FAM119A antibody LS-C102481 Western blot of Fetal Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
METTL21A / FAM119A antibody LS-C102481 Western blot of Fetal Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From:
Date Requested: 6/19/2018
Date Requested: 6/19/2018
