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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HAX‑1 Antibody (aa107‑156) LS‑C116450
HAX-1 antibody LS-C116450 is an unconjugated rabbit polyclonal antibody to HAX-1 from human, bovine, guinea pig and other species. Validated for WB.
Catalog
Size
Price
LS-C116450-50
50 µg
Unavailable

Popular HAX-1 Products

Western blot analysis of Hax1a in human brain tissue lysate with Hax1a antibody at (A) 1 and (B) 2 µg/mL.
Species: Human, Mouse, Rat
Applications: Western blot, ELISA
Immunohistochemistry of paraffin-embedded mouse heart.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Immunohistochemical analysis of HAX1 staining in mouse heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

HAX-1 antibody LS-C116450 is an unconjugated rabbit polyclonal antibody to HAX-1 from human, bovine, guinea pig and other species. Validated for WB.
About HAX-1
HAX-1 is known to associate with hematopoietic cell-specific Lyn substrate 1, a substrate of Src family tyrosine kinases. It also interacts with the product of the polycystic kidney disease 2 gene, mutations in which are associated with autosomal-dominant polycystic kidney disease, and with the F-actin-binding protein, cortactin. It was earlier thought that this gene product is mainly localized in the mitochondria, however, recent studies indicate it to be localized in the cell body. O00165 NM_006118 NP_006109.2

HAX1 Antibody, HAX-1 Antibody, HCLSBP1 Antibody, HS1BP1 Antibody, HCLS1-associated protein X-1 Antibody, HS1 binding protein Antibody, HS1-associating protein Antibody, HS1-binding protein 1 Antibody, SCN3 Antibody, HCLS1 associated protein X-1 Antibody, HS1-associating protein X-1 Antibody, HSP1BP-1 Antibody


Specifications

Target
Human HAX-1
Host
Rabbit
Reactivity
Human, Monkey, Bovine, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bat, Zebrafish (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
HAX-1 antibody was raised against synthetic peptide located between aa107-156 of human HAX1 (O00165, NP_001018238). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Elephant, Bovine, Rabbit, Horse, Pig, Guinea pig (100%); Galago, Rat (92%); Mouse, Bat (91%); Zebrafish (90%); Marmoset (85%); Platypus (83%).
Blocking Peptide
LS-E18724 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C116450. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa107-156
Specificity
Human HAX1
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

HAX1 antibody LS-C116450 Western blot of Fetal Heart lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HAX1 antibody LS-C116450 Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HAX1 antibody LS-C116450 Western blot of Fetal Heart lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HAX1 antibody LS-C116450 Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HAX1 antibody LS-C116450 Western blot of Fetal Heart lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HAX1 antibody LS-C116450 Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HAX1 antibody LS-C116450 Western blot of Fetal Heart lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HAX1 antibody LS-C116450 Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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