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Anti-HAVCR1 / KIM-1 Antibody (aa200-300) IHC-plus™ LS-B2031

Note: This antibody replaces LS-C685

Ordering

Wt. Vol. Conc. Price
50 µg 100 µl 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular HAVCR1 / KIM-1 Antibodies

Anti-HAVCR1 / KIM-1 Antibody (aa200-300) IHC-plus™ LS-B2031
Rabbit Polyclonal (IgG) to Human HAVCR1 / KIM-1
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-HAVCR1 / KIM-1 Antibody (N-Terminus) IHC-plus™ LS-B2103
Rabbit Polyclonal to Human HAVCR1 / KIM-1
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-HAVCR1 / KIM-1 Antibody (Internal) IHC-plus™ LS-B2463
Rabbit Polyclonal to Human HAVCR1 / KIM-1
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human HAVCR1 / KIM-1
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated

Details

Human HAVCR1 / KIM-1
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (2.5 - 5 µg/ml)
  • Western blot (1 - 3 µg/ml)

Specificity and Use

HAVCR1 / KIM-1 antibody was raised against a synthetic peptide (SPLYSYTTDGNDTVT) of human HAVCR1/TIM-1protein were used as immunogen. This sequence corresponds to amino acids (aa) 248-262 of NP_036338 and aa 84-98 of EAW61615.1. Isoforms of HAVCR1/TIM-1 have been described. For example, NP_036338 is 364 aa and EAW61615.1 is 194 aa. EAW61615.1 is referred to as isoform CRA_a. Percent identity by BLAST analysis: Human, Gorilla (100%); Monkey (93%); Dog (80%).
aa200-300
The amino acid sequence (SPLYSYTTDGNDTVT) used as immunogen for this is 100% homologous in human, 93% homologous in vervet/green monkey and 80% homologous in chimp. Isoforms of different molecular weights have been described for TIM-1. The sequence used for immunogen is conserved between TIM-1 (364aa, NP_036338) and TIM-1 isoform CRA_a (194aa, EAW61615.1). Therefore, the molecular weight observed in western blots may vary depending on the forms present.
Immunohistochemistry: LS-B2031 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2031 was determined to be 2.5-5 ug/ml.

Packaging

PBS, 0.05% BSA, 0.05% sodium azide.
Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.
For research use only.

About HAVCR1 / KIM-1

Q96D42 NM_012206 NP_036338.2

HAVCR1 Antibody, HAVCR-1 Antibody, KIM-1 Antibody, Kidney injury molecule 1 Antibody, KIM1 Antibody, T-cell membrane protein 1 Antibody, TIM1 Antibody, TIMD1 Antibody, TIM-1 Antibody, HAVCR Antibody, TIMD-1 Antibody

Hepatitis A Virus Cellular Receptor 1 (HAVCR1) is a membrane receptor for both human hepatitis A virus (HHAV) and TIMD4. HAVCR1 may be involved in the moderation of asthma and allergic diseases. The reference genome represents an allele that retains a MTTVP amino acid segment that confers protection against atopy in HHAV seropositive individuals.

Immunohistochemistry

Immunohistochemistry
Anti-TIM-1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2031 concentration 5 ug/ml.

Western blot

Western blot
Western blot of HAVCR1/TIM-1 in human brain lysate in the A) absence and B) presence of immunizing peptide, and C) mouse brain lysate.

Requested From: United States
Date Requested: 12/7/2016

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