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Anti-HADH Antibody (clone 4B5) IHC-plus™ LS-B6093

Note: This antibody replaces LS-C133229

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular HADH Antibodies

Anti-HADH Antibody (clone 4B5) IHC-plus™ LS-B6093
Mouse Monoclonal [clone 4B5] (IgG2b,k) to Human HADH
Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-HADH Antibody (aa234-245) IHC-plus™ LS-B11172
Goat Polyclonal to Human HADH
Human, Monkey, Bat, Dog, Horse, Pig
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-HADH Antibody (aa149-198) IHC-plus™ LS-B11524
Rabbit Polyclonal to Human HADH
Human, Monkey, Mouse, Rat, Bat, Dog, Guinea pig, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 4B5] (IgG2b,k) to Human HADH
Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human HADH
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [4B5]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
  • ELISA

Specificity and Use

HADH antibody was raised against hADHSC (NP_005318, 205 a.a. ~ 315 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human HADH
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2. Sourced in Ascites.
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
For research use only.

About HADH

Q16836 NM_005327 NP_005318.3

HADH Antibody, HAD Antibody, HADH1 Antibody, HCDH Antibody, HHF4 Antibody, SCHAD Antibody, HADHSC Antibody, Hydroxyacyl-CoA dehydrogenase Antibody, MSCHAD Antibody

SCHAD / HADHSC is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15.

Immunohistochemistry

Immunohistochemistry
Anti-HADH / HADHSC antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6093 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to HADHSC on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot
Western blot of HADH expression in HepG2 cell lysate.

Western blot

Western blot
Western blot of HADH expression in transfected 293T cell line by HADH monoclonal antibody.

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation of HADH transfected lysate using anti-HADH monoclonal antibody and Protein A Magnetic Bead.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged HADHSC is approximately 0.03 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 12/6/2016

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