LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-H2AFZ / H2A.z Antibody (aa65-158) LS-C185966

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular H2AFZ / H2A.z Antibodies

Anti-H2AFZ / H2A.z Antibody LS-C185971
Rabbit Polyclonal to Human H2AFZ / H2A.z
Human
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-H2AFZ / H2A.z Antibody (aa59-86, AP) LS-C258220
Rabbit Polyclonal (IgG) to Human H2AFZ / H2A.z
Human
IHC, Western blot, ELISA
AP Conjugated
Anti-H2AFZ / H2A.z Antibody (aa2-128, FITC) LS-C302908
Rabbit Polyclonal (IgG) to Human H2AFZ / H2A.z
Human
Western blot, ELISA
FITC Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human H2AFZ / H2A.z
Human
IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated

Details

Human H2AFZ / H2A.z
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
  • Immunoprecipitation (1:500 - 1:1000)

Specificity and Use

H2AFZ / H2A.z antibody was raised against synthetic peptide corresponding to a region within amino acids 65 and 158 of Histone H2A. Z.
aa65-158
Human H2AFZ / H2A.z
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About H2AFZ / H2A.z

P0C0S5 NM_002106 NP_002097.1

H2AFZ Antibody, H2AZ histone Antibody, H2A histone family, member Z Antibody, H2A.z Antibody, H2AZ Antibody, Histone H2A.Z Antibody

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded ES-2 xenograft using Histone H2A.Z antibody at 1:100 dilution.

Immunocytochemistry

Immunocytochemistry
Immunofluorescence of paraformaldehyde-fixed A431 using Histone H2A.Z antibody at 1:500 dilution.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: MOLT4, B: Raji. 12% SDS PAGE. H2AFZ / H2A.z antibody diluted at 1:3000

Immunoprecipitation

Immunoprecipitation
Histone H2A.Z antibody immunoprecipitates Histone H2A.Z protein in IP experiments. IP Sample:1000 ug HeLa whole cell lysate/extract A. 50 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of Histone H2A.Z protein by 2 ug of Histone H2A.Z antibody 15% SDS-PAGE The immunoprecipitated Histone H2A.Z protein was detected by Histone H2A.Z antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation
Histone H2A.Z antibody immunoprecipitates Histone H2A.Z protein-DNA in ChrIP experiments. ChrIP Sample: HeLa whole cell lysate/extract A. 5 ug preimmune rabbit IgG B. 5 ug of Histone H2A.Z antibody The precipitated DNA was detected by PCR with primer set targeting to GAPDH gene locus.
 

Requested From: United States
Date Requested: 12/9/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number