Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
H2AFZ Antibody, H2AZ histone Antibody, H2A histone family, member Z Antibody, H2A.z Antibody, H2AZ Antibody, Histone H2A.Z Antibody
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
IHC of paraffin-embedded ES-2 xenograft using Histone H2A.Z antibody at 1:100 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using Histone H2A.Z antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate). A: MOLT4, B: Raji. 12% SDS PAGE. H2AFZ / H2A.z antibody diluted at 1:3000
Histone H2A.Z antibody immunoprecipitates Histone H2A.Z protein in IP experiments. IP Sample:1000 ug HeLa whole cell lysate/extract A. 50 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of Histone H2A.Z protein by 2 ug of Histone H2A.Z antibody 15% SDS-PAGE The immunoprecipitated Histone H2A.Z protein was detected by Histone H2A.Z antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.
Histone H2A.Z antibody immunoprecipitates Histone H2A.Z protein-DNA in ChrIP experiments. ChrIP Sample: HeLa whole cell lysate/extract A. 5 ug preimmune rabbit IgG B. 5 ug of Histone H2A.Z antibody The precipitated DNA was detected by PCR with primer set targeting to GAPDH gene locus.
Requested From: United States
Date Requested: 10/24/2016