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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GZMB / Granzyme B Antibody (clone GRB‑7) LS‑C121911
Antibody
Granzyme B antibody LS-C121911 is an unconjugated mouse monoclonal antibody to human Granzyme B (GZMB). Validated for IHC and WB.
Catalog
Size
Price
LS-C121911-1000
1000 µl
Unavailable

Popular GZMB / Granzyme B Products

Antibody
Species: Human, Monkey, Rhesus monkey
Applications: IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry, ELISA
Antibody
Species: Human, Monkey, Rhesus monkey
Applications: IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry, ELISA
Anti-GZMB / Granzyme B antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2783 concentration 10 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin
Anti-GZMB / Granzyme B antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7602 dilution 1:100.
Species: Human, Mouse, Rat
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IHC of paraffin-embedded Human Kidney tissue using anti-GZMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

Product Description

Granzyme B antibody LS-C121911 is an unconjugated mouse monoclonal antibody to human Granzyme B (GZMB). Validated for IHC and WB.
About GZMB / Granzyme B
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. P10144 NM_004131 NP_004122.2

GZMB Antibody, CCPI Antibody, CGL-1 Antibody, Cathepsin G-like 1 Antibody, CSP-B Antibody, CTLA-1 Antibody, CTLA1 Antibody, CGL1 Antibody, CSPB Antibody, Fragmentin 2 Antibody, GRB Antibody, HLP Antibody, Human lymphocyte protein Antibody, Fragmentin-2 Antibody, SECT Antibody, Lymphocyte protease Antibody, T-cell serine protease 1-3E Antibody, C11 Antibody, CTSGL1 Antibody, Cytotoxic serine protease B Antibody, Granzyme B Antibody, Granzyme-2 Antibody


Specifications

Target
Human GZMB / Granzyme B
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [GRB-7]
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Western blot (1:20)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Human granzyme B.
Specificity
Recognizes the 33kD human serine protease granzyme B. Does not react with human granzyme A. It is used as a marker for NK-cells and activated cytotoxic T-cells (CTL). This protease is localized in cytoplasmic granules and gives a granular staining pattern. Granzyme B is involved in target cell apoptosis during lymphocyte mediated cyto-toxicity. Exocytosis of granzyme containing granules in the cytoplasm of the target cell will lead to induction of DNA fragmentation and apoptosis of the target cell.
Usage
Suitable for use in Western Blot and Immunohistochemistry: 1:20 Stains granzyme B expressing lymphocytes in sections of sublimate formaldehyde as well as formalin fixed paraffin embedded tissues. Does not react with other cell types. Can not be used on frozen sections. Pretreatment with 0.1 M sodium citrate, for 10 min at 100°C in a microwave oven is advised.
Presentation
1% BSA, 0.1% sodium azide
Storage
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/25/2018

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