Human GZMB / Granzyme B

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

GRB-7

Unconjugated

Tissue culture supernatant

Unmodified

Human granzyme B.

Recognizes the 33kD human serine protease granzyme B. Does not react with human granzyme A. It is used as a marker for NK-cells and activated cytotoxic T-cells (CTL). This protease is localized in cytoplasmic granules and gives a granular staining pattern. Granzyme B is involved in target cell apoptosis during lymphocyte mediated cyto-toxicity. Exocytosis of granzyme containing granules in the cytoplasm of the target cell will lead to induction of DNA fragmentation and apoptosis of the target cell.

• IHC
• IHC - Paraffin
• IHC - Frozen
• Western blot (1:20)

Suitable for use in Western Blot and Immunohistochemistry: 1:20 Stains granzyme B expressing lymphocytes in sections of sublimate formaldehyde as well as formalin fixed paraffin embedded tissues. Does not react with other cell types. Can not be used on frozen sections. Pretreatment with 0.1 M sodium citrate, for 10 min at 100°C in a microwave oven is advised.

0.02% Sodium Azide, 0.1% BSA

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P10144 NCBI: NM_004131 NP_004122.2