Human GZMB / Granzyme B
(tested or 100% immunogen sequence identity)
Tissue culture supernatant
- IHC - Paraffin
- IHC - Frozen
- Western blot (1:20)
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Human granzyme B.
Recognizes the 33kD human serine protease granzyme B. Does not react with human granzyme A. It is used as a marker for NK-cells and activated cytotoxic T-cells (CTL). This protease is localized in cytoplasmic granules and gives a granular staining pattern. Granzyme B is involved in target cell apoptosis during lymphocyte mediated cyto-toxicity. Exocytosis of granzyme containing granules in the cytoplasm of the target cell will lead to induction of DNA fragmentation and apoptosis of the target cell.
Suitable for use in Western Blot and Immunohistochemistry: 1:20 Stains granzyme B expressing lymphocytes in sections of sublimate formaldehyde as well as formalin fixed paraffin embedded tissues. Does not react with other cell types. Can not be used on frozen sections. Pretreatment with 0.1 M sodium citrate, for 10 min at 100°C in a microwave oven is advised.
1% BSA, 0.1% sodium azide
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
For research use only.
This antibody carries the LSBio 100% Guarantee.