Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
GRM5 / MGLUR5 antibody was raised against synthetic peptide derived from the C-terminal region of the mGluR5 and mGluR1a.
Reacts with the ~125kD mGluR5, ~125kD mGluR1a and ~250kD mGluR5 and mGluR1a dimers. Antibody reactivity was blocked by preabsorption of antibody with peptide immunogen. Reactivity has been confirmed using rat brain.
Suitable for use in Western Blot. Western Blot: 1:1000.
200 mM Tris/glycine buffer, pH 7.5, 0.1% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
Group I of the Metabotropic Glutamate Receptors includes GRM1 and GRM5, receptors that have been shown to activate phospholipase C. Alternative splice variants of GRM5 have been described, but their full-length nature has not been determined. Inflammation results in the activation of mGluR1 and mGluR5 in dorsal horn neurons of the spinal cord, leading to extracellular signal-regulated kinase (ERK) activation, which is required for nociceptive plasticity and enhanced pain.