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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GRIN2B / NMDAR2B / NR2B Antibody (phospho‑Tyr1474) LS‑C199332
NR2B antibody LS-C199332 is an unconjugated rabbit polyclonal antibody to NR2B (GRIN2B / NMDAR2B) from human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C199332-50
50 µl
Unavailable
LS-C199332-100
100 µl
Unavailable

Popular GRIN2B / NMDAR2B / NR2B Products

Western blot of 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~180k NR213 subunit of the NMDA receptor.
Species: Rat, Human, Mouse
Applications: IHC, IHC - Frozen, Western blot
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Species: Mouse, Human, Monkey, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Applications: Western blot, ELISA
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Applications: IHC, IHC - Paraffin, ICC, Western blot
Anti-GRIN2B / NMDAR2B antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7462 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rat, Human, Mouse
Applications: IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation

Product Description

NR2B antibody LS-C199332 is an unconjugated rabbit polyclonal antibody to NR2B (GRIN2B / NMDAR2B) from human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
About GRIN2B / NMDAR2B / NR2B
N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. Q13224 NM_000834 NP_000825.2

GRIN2B Antibody, GluN2B Antibody, GluR epsilon 2 Antibody, NMDA receptor 2B Antibody, NMDAR2B Antibody, NR2B Antibody, NR3 Antibody, MRD6 Antibody, HNR3 Antibody


Specifications

Target
Human GRIN2B / NMDAR2B / NR2B
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide derived from human NMDAR2B around the phosphorylation site of Tyr1474.
Epitope
pTyr1474
Specificity
NMDAR2B (Phospho-Tyr1474) Antibody detects endogenous levels of NMDAR2B only when phosphorylated at tyrosine1474.
Presentation
PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded human brain, using NMDAR2B (Phospho-Tyr1474) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human brain, using NMDAR2B (Phospho-Tyr1474) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from Jurkat cells treated with UV 15', using NMDAR2B (Phospho-Tyr1474) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from Jurkat cells treated with UV 15', using NMDAR2B (Phospho-Tyr1474) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human brain, using NMDAR2B (Phospho-Tyr1474) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human brain, using NMDAR2B (Phospho-Tyr1474) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from Jurkat cells treated with UV 15', using NMDAR2B (Phospho-Tyr1474) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from Jurkat cells treated with UV 15', using NMDAR2B (Phospho-Tyr1474) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human brain, using NMDAR2B (Phospho-Tyr1474) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human brain, using NMDAR2B (Phospho-Tyr1474) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from Jurkat cells treated with UV 15', using NMDAR2B (Phospho-Tyr1474) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from Jurkat cells treated with UV 15', using NMDAR2B (Phospho-Tyr1474) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human brain, using NMDAR2B (Phospho-Tyr1474) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human brain, using NMDAR2B (Phospho-Tyr1474) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from Jurkat cells treated with UV 15', using NMDAR2B (Phospho-Tyr1474) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from Jurkat cells treated with UV 15', using NMDAR2B (Phospho-Tyr1474) Antibody. The lane on the right was incubated with synthetic peptide.


Requested From: 
Date Requested: 6/18/2018

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