Target
Rat GRIN1 / NMDAR1
Synonyms
GRIN1 | GluN1 | NMDAR1 | NR1 | MRD8 | NMD-R1 | NMDA receptor A1 | NMDA1 | NR1a | GluR zeta 1 | N-methyl-D aspartate receptor
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
Fusion protein of aa1-564 from rat NR1.
Specificity
Recognizes rat NMDA receptor subunit 1 (all splice variants). No cross-reactivity observed with other NMDA receptor subunits. Recognizes a 120kD protein in rat brain hippocampal homogenate by Western Blot.
Applications
- Western blot (1:300)
- Immunoprecipitation
Usage
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:300. It is recommended that the antibody be diluted in TBST (20 mM Tris-HCl, 137 mM NaCl, 1% Tween-20, pH 7.6) containing 5% nonfat milk and 0.05% sodium azide. Immunoprecipitation: 3 ug per 200 ug lysate.
Reconstitution
Supplied as a lyophilized powder in DMEM containing fetal bovine serum, no preservative. Reconstitute with 50ul sterile 40-50% glycerol, PBS.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee